Results 201 to 210 of about 2,021,170 (319)

Large-scale evaluation of HIV-1 DNA drug resistance testing as a robust tool for clinical decision-making: A nationwide study in China. [PDF]

J Pharm Anal
Wu C, Zhang L, Chen Z, Ma W, Fu Y, Yang K, Liu M, Li Y, Chen X, Hou M, Liu M, Deng A, Zhao Q, Zhang L, Wang Q, Peng J, Li Y, Deng K, Bai J, Long H, Chen Y, Wang H, He Y, Li J, Guo J, Cao B, Cui Y, Wang M, Zhu T, Yao J, Wang T.   +30 more
europepmc   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Standardizing classification and assessment methods in clinical frailty scale evaluation for elderly trimalleolar fractures. [PDF]

J Orthop Surg Res
Wang JW, Liu JH, Dai MW.
europepmc   +1 more source

Exon 7 splicing of ERα predicts poor prognosis and increases phenotypic heterogeneity in luminal a subtype breast cancer

FEBS Open Bio, EarlyView.
ERα splice variant ERα∆7 lacks the C‐terminus, and its expression may change phenotypes of breast cancers. Our results showed that ERα∆7 is found in the luminal A subtype, and elevated ERα∆7 levels are linked to improved cell survival with lower proliferation and migration.
Long Wai Tsui, Taobo Hu, Thomson Christian Husein, Wai Hei Shek, Bingrong Chen, Depeng Wang, Shu Wang, Weichuan Yu, Xiaodan Fan, Mengping Long, Toyotaka Ishibashi   +10 more
wiley   +1 more source

AI-Driven Optimization of Cu₂O Modified Bitumen: A Multi-Scale Evaluation of Rheological, Aging, and Moisture Susceptibility Performance. [PDF]

Materials (Basel)
Karahancer S.
europepmc   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Large-scale evaluation of soybean germplasm reveals geographic patterns in shade tolerance and identifies elite genotypes for intercropping systems. [PDF]

BMC Plant Biol
Zhang F, Hong H, Liu X, Wang X, Zhang C, Zhao K, Yuan R, Abdelghany AM, Zhang B, Lamlom SF, Ren H.   +10 more
europepmc   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Cross-cultural validation of the profile of mood scale: evaluation of the psychometric properties of short screening versions. [PDF]

Front Psychol
Schmalbach I, Schmalbach B, Aghababa A, Brand R, Chang YK, Çiftçi MC, Elsangedy H, Fernández Gavira J, Huang Z, Kristjánsdóttir H, Mallia L, Nosrat S, Pesce C, Rafnsson D, Medina Rebollo D, Timme S, Brähler E, Petrowski K.   +17 more
europepmc   +1 more source

Matrix metalloproteinase‐9 regulates cell adhesion and membrane protrusive activity of ovarian cancer cells

FEBS Open Bio, EarlyView.
Matrix metalloproteinase‐9 (MMP9) drives ovarian cancer progression. Using MMP9‐null cells (M9‐KO) created from ovarian cancer cells, we found MMP9 loss did not block Epidermal Growth Factor (EGF)‐driven E‐cadherin dissolution or EMT but delayed and reduced EGF‐driven membrane protrusions. Transient MMP9 re‐expression drove membrane protrusion.
Claire Strauel, Sam Bennett, Natalie Ehlinger, Nelson Cook, Katelyn Marvin, Caleb L. Lines, Sam Reyes, Brooke Kirby, Shawn M. Ellerbroek   +8 more
wiley   +1 more source