Results 141 to 150 of about 3,374,770 (347)
Scalable Deep Multi-Agent Reinforcement Learning via Observation Embedding and Parameter Noise [PDF]
, 2019 Jian Zhang +3 more
openalex +1 more source
FEBS Open Bio, EarlyView.Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei +3 more
wiley +1 more source
High-yield astaxanthin production process development and scale-up validation from wild-type Phaffia rhodozyma via parameter optimization and LSTM modeling [PDF]
Pisin Chen +6 more
openalex +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
A Deep‐Learning Based Parameter Inversion Framework for Large‐Scale Groundwater Models
Geophysical Research LettersHydrogeologic models generally require gridded subsurface properties, however these inputs are often difficult to obtain and highly uncertain. Parametrizing computationally expensive models where extensive calibration is computationally infeasible is a ...
Amanda Triplett +4 more
doaj +1 more source
FEBS Open Bio, EarlyView.Amino acids sequence of two different proteins with the same sequence (chameleon sequence—black boxes) represent in 3D structure of the proteins different secondary structures: HHHH—helical and BBB—Beta‐structural. The chains folded in water environment adopt different III‐order structures in which the chameleon fragments appear to adopt similar status
Irena Roterman +4 more
wiley +1 more source
FEBS Open Bio, EarlyView.This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill +4 more
wiley +1 more source
Screening and epitope characterization of Nidogen‐2‐specific nanobodies
FEBS Open Bio, EarlyView.Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
On Double Stage Shrinkage-Bayesian Estimator for the Scale Parameter of Exponential Distribution
Ibn Al-Haitham Journal for Pure and Applied Sciences, 2017 This paper is concerned with Double Stage Shrinkage Bayesian (DSSB) Estimator for lowering the mean squared error of classical estimator ˆ q for the scale parameter (q) of an exponential distribution in a region (R) around available prior knowledge ...
A. N. Salman, M. D. Salman
doaj
Digital twins to accelerate target identification and drug development for immune‐mediated disorders
FEBS Open Bio, EarlyView.Digital twins integrate patient‐derived molecular and clinical data into personalised computational models that simulate disease mechanisms. They enable rapid identification and validation of therapeutic targets, prediction of drug responses, and prioritisation of candidate interventions.
Anna Niarakis, Philippe Moingeon
wiley +1 more source