Results 31 to 40 of about 70,957 (253)

Malate transport in Schizosaccharomyces pombe [PDF]

open access: yesJournal of Bacteriology, 1986
The transport of malate was studied in a Schizosaccharomyces pombe wild-type strain and in mutant strains unable to utilize malic acid. Two groups of such mutants, i.e., malic enzyme-deficient and malate transport-defective mutants, were differentiated by a 14C-labeled L-malate transport assay and by starch gel electrophoresis followed by activity ...
C, Osothsilp, R E, Subden
openaire   +2 more sources

Schizosaccharomyces pombe Pol II transcription elongation factor ELL functions as part of a rudimentary super elongation complex [PDF]

open access: yes, 2018
ELL family transcription factors activate the overall rate of RNA polymerase II (Pol II) transcription elongation by binding directly to Pol II and suppressing its tendency to pause.
Alonso-Nunez   +54 more
core   +1 more source

Transformation of Schizosaccharomyces japonicus [PDF]

open access: yesCold Spring Harbor Protocols, 2017
This protocol describes the use of electroporation to transform Schizosaccharomyces japonicus with plasmids or linear DNA. Plasmids are helpful for the complementation testing of mutations and for the expression of specific genes. Linear DNA fragments integrated into chromosomal DNA by homologous recombination are useful for gene deletion or to fuse a ...
Keita, Aoki, Hironori, Niki
openaire   +2 more sources

Determinants of RPA megafoci localization to the nuclear periphery in response to replication stress

open access: yesG3: Genes, Genomes, Genetics, 2022
Upon replication stress, ssDNA, coated by the ssDNA-binding protein RPA, accumulates and generates a signal to activate the replication stress response. Severe replication stress induced by the loss of minichromosome maintenance helicase subunit Mcm4 in ...
Seong Min Kim, Susan L Forsburg
doaj   +1 more source

The role of Schizosaccharomyces pombe SUMO ligases in genome stability [PDF]

open access: yes, 2007
SUMOylation is a post-translational modification that affects a large number of proteins, many of which are nuclear. While the role of SUMOylation is beginning to be elucidated, it is clear that understanding the mechanisms that regulate the process is ...
A. Skilton   +44 more
core   +2 more sources

Peptide Variant Detection by a Living Yeast Biosensor via an Epitope-Selective Protease

open access: yesBioDesign Research, 2023
We previously demonstrated that we could hijack the fungal pheromone signaling pathway to provide a living yeast biosensor where peptide biomarkers were recognized by G-protein-coupled receptors and engineered to transcribe a readout.
Tea Crnković   +3 more
doaj   +1 more source

Gene expression profiling data of Schizosaccharomyces pombe under nitrosative stress using differential display

open access: yesData in Brief, 2016
Excess production of nitric oxide (NO) and reactive nitrogen intermediates (RNIs) causes nitrosative stress on cells. Schizosaccharomyces pombe was used as a model to study nitrosative stress response.
Pranjal Biswas   +2 more
doaj   +1 more source

Cell wall polysaccharides released during the alcoholic fermentation by Schizosaccharomyces pombe and S. japonicus: quantification and characterization.

open access: yesFood microbiology, 2017
The present work demonstrates that yeasts belonging to the Schizosaccharomyces genus release a high quantity of polysaccharides of cell wall origin starting from the onset of the alcoholic fermentation.
P. Domizio, Y. Liu, L. Bisson, D. Barile
semanticscholar   +1 more source

Smc5/6: a link between DNA repair and unidirectional replication? [PDF]

open access: yes, 2008
Of the three structural maintenance of chromosome (SMC) complexes, two directly regulate chromosome dynamics. The third, Smc5/6, functions mainly in homologous recombination and in completing DNA replication.
A Losada   +46 more
core   +1 more source

Nanoscale architecture of the Schizosaccharomyces pombe contractile ring

open access: yeseLife, 2017
The contractile ring is a complex molecular apparatus which physically divides many eukaryotic cells. Despite knowledge of its protein composition, the molecular architecture of the ring is not known.
Nathan A. McDonald   +4 more
semanticscholar   +1 more source

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