Results 181 to 190 of about 33,254 (223)

Switching genes in Schizosaccharomyces pombe

Current Genetics, 1985
In homothallic (h 90) Schizosaccharomyces pombe strains mutants occur which exhibit reduced frequencies of mating-type switching. The colonies of such mutants show a mottled iodine reaction. The underlying mutations map either in a switching signal at matl or in switching (swi) genes which are not linked to the mating-type region.
H, Gutz, H, Schmidt
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Schizosaccharomyces pombe Isolation Protocol

2018
This chapter describes a methodology to isolate yeast strains from Schizosaccharomyces pombe species. The method is based on a selective-differential medium that notably facilitates the isolation of S. pombe. The main difficulty in isolating microorganisms from this genus is their extremely low incidence in nature when they are compared to other ...
Ángel, Benito, Fernando, Calderón, Santiago, Benito   +2 more
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Selecting Schizosaccharomyces pombe Diploids

Cold Spring Harbor Protocols, 2017
Here we describe procedures for the selection of diploid Schizosaccharomyces pombe. ade6-M210/ade6-M216 heteroallelic complementation is widely used to select for Ade+ diploids. Such diploids will readily sporulate when starved of nitrogen. For some investigations, stable diploids are preferable (e.g., for genetic complementation tests), and in these ...
Ekwall, Karl, Thon, Genevieve
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Molecular Genetics of Schizosaccharomyces pombe

2010
In this chapter we present basic protocols for the use of Schizosaccharomyces pombe, commonly known as fission yeast, in molecular biology and genetics research. Fission yeast is an increasingly popular model organism for the study of biological pathways because of its genetic tractability and as a model for metazoan biology. It provides an alternative
Sarah A, Sabatinos, Susan L, Forsburg
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Cell cycle regulation in Schizosaccharomyces pombe

Current Opinion in Microbiology, 2000
Cdc2, a cyclin-dependent kinase, controls cell cycle progression in fission yeast. New details of Cdc2 regulation and function have been uncovered in recent studies. These studies involve cyclins that associate with Cdc2 in G1-phase and the proteins that regulate inhibitory phosphorylation of Cdc2 during S-phase and G2-phase. Recent investigations have
B A, Moser, P, Russell
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Pac1 Ribonuclease of Schizosaccharomyces pombe

2001
Publisher Summary The Pac1 RNase of the fission yeast Schizosaccharomyces pombe is a doublestrand-specific endoribonuclease (dsRNase) whose structure, biochemical properties, and biological functions place it in the RNase III family. This chapter describes the methods that have been used to define the biological functions and biochemical properties ...
G, Rotondo, D, Frendewey
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X-ray tomography of Schizosaccharomyces pombe

Differentiation, 2007
The genetic tractability of the unicellular yeast Schizosaccharomyces pombe has resulted in it becoming an important model organism for the study of many eukaryotic cellular processes, in particular cell division. Over the past few years much progress has been made toward understanding the mechanisms that regulate eukaryotic cell division and the ...
Weiwei, Gu, Laurence D, Etkin, Mark A, Le Gros, Carolyn A, Larabell   +3 more
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Overview of Schizosaccharomyces pombe

Current Protocols in Molecular Biology, 2003
AbstractThe fission yeast S. pombe provides an attractive alternative system to budding yeast S. cerevisiae for studies of fundamental cell biology. Fission yeast is a particularly powerful model for studies of cell cycle, chromosome dynamics, and polarity. Other areas of study are also expanding.
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Characterization of Schizosaccharomyces pombe secreted proteins

HEREDITAS, 2007
Secreted proteins play a vital physiological role. Schizosaccharomyces pombe is an important model organism for cell cycle study and a potential useful drug screen model. Secreted proteins also initiate the mating. However, few global studies concerning the secreted proteins of S. pombe was reported.
Yu-Ling, Liu, Yun-Fan, Liu, Jian-Ping, Xie   +2 more
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Choosing and using Schizosaccharomyces pombe plasmids

Methods, 2004
A wide range of plasmids has been developed for molecular studies in the fission yeast Schizosaccharomyces pombe. This includes general purpose episomes, expression vectors, epitope tagging plasmids, and integration vectors. This review describes the typical features of S.
Rania, Siam, William P, Dolan, Susan L, Forsburg   +2 more
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