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Yptl (Schizosaccharomyces pombe)
1995Abstract The ypt1 gene was isolated fortuitously by hybridizing a S. pombe genomic library with a probe from the sec4 gene of 5. cerevisiae (Fawell et al. 1989) and independently with a ypt3 probe (Miyake and Yamamoto 1990). The sequence, including four introns, is given in Fig. 1(A).
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Genetic Analysis of Schizosaccharomyces pombe
Cold Spring Harbor Protocols, 2017In this introduction we discuss some basic genetic tools and techniques that are used with the fission yeast Schizosaccharomyces pombe. Genes commonly used for selection or as reporters are discussed, with an emphasis on genes that permit counterselection, intragenic complementation, or colony-color assays. S. pombe is most stable as a haploid organism.
Ekwall, Karl, Thon, Genevieve
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Schizosaccharomyces pombe Biotechnological Applications in Winemaking
2018The traditional way of producing wine is through the use of Saccharomyces cerevisiae in order to convert glucose and fructose into alcohol. In the case of red wines, after this alcoholic fermentation lactic bacteria Oenococus oeni is used to stabilize wine from a microbiological point of view by converting malic acid into lactic acid that it is not a ...
Ángel, Benito +2 more
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Putative frameshift suppressors in Schizosaccharomyces pombe
Current Genetics, 1981Nine genetically distinct suppressors of ICR-170-induced ade6 and ade7 mutations have been identified in Schizosaccharomyces pombe. The nine suppressors of ICR-170-induced and spontaneous origin have been assigned to the three chromosomes by haploidization and meiotic analysis.
H, Hottinger, U, Leupold
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Analysis of Schizosaccharomyces pombe Meiosis
Cold Spring Harbor Protocols, 2017Meiosis is a specialized cell cycle that generates haploid gametes from diploid cells. The fission yeast Schizosaccharomyces pombe is one of the best model organisms for studying the regulatory mechanisms of meiosis. S. pombe cells, which normally grow in the haploid state, diploidize by conjugation and initiate meiosis when starved for nutrients ...
Akira, Yamashita +3 more
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Schizosaccharomyces pombe minimum genome factory
Biotechnology and Applied Biochemistry, 2007Various systems for the production of useful proteins have been developed using the fission yeast Schizosaccharomyces pombe as a host, and some are now being used commercially. It is necessary, however, to improve the system further for the production of low‐cost chemicals and commodities, so that the host becomes more economical and productive and can
Yuko, Giga-Hama +3 more
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Cytokinesis in fission yeast Schizosaccharomyces pombe
1997Publisher Summary This chapter discusses the cytokinesis in fission yeast Schizosaccharomyces pombe. The process of cytokinesis in animal cells has been the subject of a large number of ingenious embryological experiments. While these experiments have revealed a great deal at a mechanistic level about what factors influence cleavage furrow formation,
M K, Balasubramanian +2 more
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Radiation resistance in Schizosaccharomyces pombe
Molecular Microbiology, 1991SummaryThe fission yeast Schizosaccharomyces pombe serves as an excellent alternative and complementary model system for the analysis of genes and gene products involved in DNA repair. This brief review outlines the advantages of S. pombe and describes the radiation‐sensitive mutants available for the analysis of DNA repair and recombination mechanisms
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