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Analysis of Schizosaccharomyces pombe Meiosis.
Cold Spring Harbor Protocols, 2017Meiosis is a specialized cell cycle that generates haploid gametes from diploid cells. The fission yeast Schizosaccharomyces pombe is one of the best model organisms for studying the regulatory mechanisms of meiosis. S.
A. Yamashita +3 more
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Overview of Schizosaccharomyces pombe
Current Protocols in Molecular Biology, 2003AbstractThe fission yeast S. pombe provides an attractive alternative system to budding yeast S. cerevisiae for studies of fundamental cell biology. Fission yeast is a particularly powerful model for studies of cell cycle, chromosome dynamics, and polarity. Other areas of study are also expanding.
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Molecular Genetics of Schizosaccharomyces pombe
2010In this chapter we present basic protocols for the use of Schizosaccharomyces pombe, commonly known as fission yeast, in molecular biology and genetics research. Fission yeast is an increasingly popular model organism for the study of biological pathways because of its genetic tractability and as a model for metazoan biology. It provides an alternative
Susan L. Forsburg, Sarah A. Sabatinos
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Pac1 Ribonuclease of Schizosaccharomyces pombe
2001Publisher Summary The Pac1 RNase of the fission yeast Schizosaccharomyces pombe is a doublestrand-specific endoribonuclease (dsRNase) whose structure, biochemical properties, and biological functions place it in the RNase III family. This chapter describes the methods that have been used to define the biological functions and biochemical properties ...
David Frendewey, G. Rotondo
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Genetic Analysis of Schizosaccharomyces pombe
Cold Spring Harbor Protocols, 2017In this introduction we discuss some basic genetic tools and techniques that are used with the fission yeast Schizosaccharomyces pombe. Genes commonly used for selection or as reporters are discussed, with an emphasis on genes that permit counterselection, intragenic complementation, or colony-color assays. S. pombe is most stable as a haploid organism.
Ekwall, Karl, Thon, Genevieve
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Cnb1 involved in cytokinesis in Schizosaccharomyces pombe
Hereditas (Beijing), 2013Serine/Threonine-specific calcineurin (CN) is highly conserved in eukaryotes, which plays an important role in transcriptional regulation. In Schizosaccharomyces pombe, CN exists as a heterodimer composed by catalytic subunit Ppb1 and regulatory subunit Cnb1.
Bi-Wei Feng +5 more
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CSL proteins of Schizosaccharomyces pombe
2009The CSL family of transcription factors is essential for metazoan development,mostly due to their involvement in the Notch signaling pathway. We identified two novel classes of CSL genes in several fungal species, organisms lacking the Notch pathway. We characterized experimentally cbfl I- and cbfl2*, the two CSL genesof Schizosaccharomyces pombe, in ...
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The ypt proteins of Schizosaccharomyces pombe
Biochemical Society Transactions, 199465 66 Mayer, B. J., Ren, K., Clark, K. I,. and Baltimore, D. (1993) Cell 73,629-630 Haslarn, K. J., Koide, H. B. and Hemmings, B. A. (1993) Nature (London) 363,309-310 Musacchio, A., Gibson, T., Price, P., Thompson, J. and Saraste, M. (1993) Trends Biochem. Sci. 18, 343-348 Lefkowitz, R. J. (1993) Cell 74,409-412 Bourne, H. K., Sanders, D. A.
Everton Robinson +5 more
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New applications for Schizosaccharomyces pombe in the alcoholic fermentation of red wines
, 2012The fermentation of grape must using nonâSaccharomyces yeasts with particular metabolic and biochemical properties is of growing interest. In the present work, red grape must was fermented using four strains of Schizosaccharomyces pombe (935, 936, 938 ...
S. Benito +4 more
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Cell cycle regulation in Schizosaccharomyces pombe
Current Opinion in Microbiology, 2000Cdc2, a cyclin-dependent kinase, controls cell cycle progression in fission yeast. New details of Cdc2 regulation and function have been uncovered in recent studies. These studies involve cyclins that associate with Cdc2 in G1-phase and the proteins that regulate inhibitory phosphorylation of Cdc2 during S-phase and G2-phase. Recent investigations have
Paul Russell, Bettina A. Moser
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