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Yeast ribosomal proteins: VII. Cytoplasmic ribosomal proteins from Schizosaccharomyces pombe
Molecular and General Genetics MGG, 1983The cytoplasmic ribosomal proteins from a fission yeast Schizosaccharomyces pombe were analysed by two-dimensional polyacrylamide gel electrophoresis. Seventy-three protein species were identified in the 80S ribosome, and named SP-S1 to SP-S33 and SP-L1 to SP-L40 in the small and large subunits, respectively.
E, Otaka, K, Higo, T, Itoh
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Yeast, 1992
AbstractA technique is described for the isolation and purification of intact, respiratory‐competent mitochondria from Sachizo‐saccharomyces pombe. The purified mitochondria are capabel of oxidizing NADH and succinate as resporatory substrates. indicating the presence of succinate dehydorgenase and an NADH dehydrogenase located on the outer suface of ...
A L, Moore +4 more
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AbstractA technique is described for the isolation and purification of intact, respiratory‐competent mitochondria from Sachizo‐saccharomyces pombe. The purified mitochondria are capabel of oxidizing NADH and succinate as resporatory substrates. indicating the presence of succinate dehydorgenase and an NADH dehydrogenase located on the outer suface of ...
A L, Moore +4 more
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Recombinational repair in Schizosaccharomyces pombe: The role of mediator proteins
Molecular Biology, 2012Repair of double-stranded DNA breaks that occur spontaneously or under the influence of external factors, are critical for cell survival. Evolutionarily conserved mechanism for error-free recombinational repair plays a major role in maintaining genome integrity.
O S, Khasanova +2 more
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Vectors for the expression of tagged proteins in Schizosaccharomyces pombe
Gene, 1998A series of vectors is described which enables the episomal expression of proteins fused to different tag sequences in Schizosaccharomyces pombe. Proteins can be expressed with their amino termini fused to GFP/EGFP, three copies of the HA or Pk epitopes or a combined tag which contains two copies of the myc epitope and six histidine residues (MH ...
Craven, R A +5 more
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CSL proteins of Schizosaccharomyces pombe
2009The CSL family of transcription factors is essential for metazoan development,mostly due to their involvement in the Notch signaling pathway. We identified two novel classes of CSL genes in several fungal species, organisms lacking the Notch pathway. We characterized experimentally cbfl I- and cbfl2*, the two CSL genesof Schizosaccharomyces pombe, in ...
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Method for protein tagging in Schizosaccharomyces pombe.
Revista medico-chirurgicala a Societatii de Medici si Naturalisti din Iasi, 2007Tagging is a useful method for the investigation of proteins. It allows the localization of the proteins in the cell, their purification in order to investigate their function and the determination of their expression. The aim of the present study was to tag the Rad32 protein of fission yeast (which is the homologue of Mre11 protein from humans) at its
Elena, Petrescu-Dănilă +5 more
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Schizosaccharomyces pombe fragile mutants as a host for heterologous protein production
Journal of Biotechnology, 1997In order to gain information about the potential interest of the Schizosaccharomyces pombe srb 1 fragile mutants as a host for heterologous protein production, the extracellular secretion of homologous and heterologous invertases was investigated. Under catabolic derepression the fragile srb 1 mutants released into the extracellular medium 5-6-fold ...
V, Zárate, F, Belda
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Primary structure of the ribosomal protein gene S6 from Schizosaccharomyces pombe
Current Genetics, 1988We have determined the nucleotide sequence of a ribosomal protein gene which codes for the ribosomal protein S6 (rps6). The sequence analysis revealed that the gene comprises 239 amino acids, giving rise to a basic protein with a molecular weight of 27,502 Da.
T, Gross +4 more
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Characterization of Nrl1 protein in Schizosaccharomyces pombe
2016The fission yeast Schizosaccharomyces pombe, is an ideal model organism to study eukaryotic cell biology as it is easy to manipulate while sharing many features in common with higher organisms like centromeres with repetitive sequences, large replication origins, conserved telomere proteins, conserved heterochromatin proteins and epigenetic silencing ...
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Cold Spring Harbor Protocols, 2017
Schizosaccharomyces pombe is an attractive model organism with which to study core principles of conserved molecular cell biology processes. The ability to monitor protein behavior following separation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) underpins much of this activity.
Grallert, Agnes, Hagan, Iain M.
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Schizosaccharomyces pombe is an attractive model organism with which to study core principles of conserved molecular cell biology processes. The ability to monitor protein behavior following separation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) underpins much of this activity.
Grallert, Agnes, Hagan, Iain M.
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