Results 151 to 160 of about 659,404 (273)

Protein Diffusion in Electroporation‐Based Biopsy: Quantifying Protein Transport From Human Skin for Skin Liquid Sampling With Electroporation

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Electroporation‐based biopsy (e‐biopsy) enables minimally invasive extraction of tissue proteins without excision; however, the effective diffusion from tissue into the sampling medium, a key parameter required for this procedure, has not been quantified in human tissues. We coupled time‐resolved aqueous sampling with a one‐dimensional Fickian
Amir Duenyas, Batel Gabay, Ariel Berl, Ofir Shir‐Az, Avshalom Shalom, Martin L. Yarmush, Alexander Golberg   +6 more
wiley   +1 more source

Optimizing Pichia Pastoris Cell‐Free Protein Synthesis to Improve Economics

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Cell‐free protein synthesis (CFPS) is a powerful and versatile platform that supports a wide range of applications, from fundamental studies of the genetic code to scalable and rapid protein production. The recently developed Pichia pastoris CFPS combines advantages of both prokaryotic and eukaryotic systems, including a rapid growth rate ...
Rui Wu, Igor Koop, Gavin Murugan, Thagshanen Thillairajah, Karen M. Polizzi   +4 more
wiley   +1 more source

Research on Training Mode of Civil Engineering Professionals Based on School-enterprise Cooperation

Journal of International Education and Development, 2022
Min Yan, Juan Du
openaire   +1 more source

Addressing Small Data Challenges in Biopharmaceutical Development and Manufacturing: A Mini Review of Multi‐Fidelity Techniques

Biotechnology and Bioengineering, EarlyView.
ABSTRACT The growing demand for biopharmaceutical products reflects their effectiveness in medical treatments. However, developing new biopharmaceuticals remains a major bottleneck, often taking up to a decade before market approval. Machine learning (ML) models have the potential to accelerate this process, but their success depends on access to large
Mohammad Golzarijalal, Uwe Aickelin, Ellen Otte   +2 more
wiley   +1 more source

One‐Pot Synthesis of a Small Synthetic Ligand for Antibody Purification

Biotechnology and Bioengineering, EarlyView.
One‐pot liquid‐phase synthesis of the synthetic ligand B1Al2A2 enables controllable ligand density and efficient capture of IgG and IgY with high recovery and purity, streamlining small‐ligand affinity purification workflows. ABSTRACT The development of synthetic affinity ligands offers a cost‐effective alternative to traditional biological ligands ...
Carolina Mota Natal, Catarina Domingos, André Nascimento, José Mendes, Afonso B. Ruiz, Ana Margarida Gonçalves Carvalho Dias, Luisa M. Ferreira, Cristina Peixoto, Ana Cecília Afonso Roque   +8 more
wiley   +1 more source

Teaching Practice to Improve the Laboratory Strategy of the Hydraulic Course at the School of Civil Engineering

, 2022
Brayan Dubán Laiton Suárez
openalex   +1 more source

Machine Learning Models of Phase Contrast Images Predict Efficiency of Human Pluripotent Stem Cell Differentiation to Cardiomyocytes

Biotechnology and Bioengineering, EarlyView.
Human pluripotent stem cells were differentiated to cTnT+ cardiomyocytes and imaged throughout the process using phase contrast. We predicted differentiation efficiency (cTnT+ area) from phase contrast and compared model performance with a live‐cell cTnT‐GFP reporter.
Austin K. Feeney, Yaniv Ovadia, Aaron D. Simmons, Christian Elabd, Claire J. Peplinski, Frank Li, Sean P. Palecek   +6 more
wiley   +1 more source

IMACulate(DE3), an E. coli Strain for High Purity His‐Tagged Protein Purifications

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Immobilised Metal Affinity Chromatography (IMAC) is widely used to purify his‐tagged recombinant proteins from Escherichia coli. However, endogenous contaminants with histidine clusters, such as GFAT and PDH E1 proteins, are often co‐purified with the target protein.
Jia Q. Truong, Dingyi Yu, Nan Hao, Chris Langendorf, Keith. E. Shearwin, Jessica K. Holien   +5 more
wiley   +1 more source

EAH221 - MEKANIK BENDALIR UNTUK JURUTERA AWAM. OKTOBER 2003 [PDF]

, 2003
PPKAW, Pusat Pengajian Kejuruteraan Awam (School of Civil Engineering)
core  

Roles of 2D halide perovskites in 2D/3D heterojunctions for high‐performance perovskite solar cells

Bulletin of the Korean Chemical Society, EarlyView.
This review establishes a design framework for two‐dimensional (2D)/three‐dimensional (3D) perovskite heterojunctions, redefining the 2D perovskite layer as an active semiconductor component. We classify 2D structures by spacer chemistry and analyze their roles within the heterojunction.
Seungmin Lee, Oui Jin Oh, Dong Hyun Kim, Dong Hun Kang, Jun Hong Noh   +4 more
wiley   +1 more source