Results 121 to 130 of about 38,116,695 (254)
Protocol for quantifying miRNA trafficking across the endosomal membrane
FEBS Open Bio, EarlyView.An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Engineers Without Borders - Guatemala - 2013 - 054
, 2019 The Carroll College Chapter of Engineers Without Borders USA is dedicated to improving the quality of life for people in developing communities worldwide through sustainable community development projects, and preparing future leaders who are well ...
Weight, Willis +1 more
core
Heterotropic regulation and negative homotropic cooperativity
FEBS Open Bio, EarlyView.We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea +5 more
wiley +1 more source
Engineers Without Borders - Guatemala - 2015 - 240
, 2019 The Carroll College Chapter of Engineers Without Borders USA is dedicated to improving the quality of life for people in developing communities worldwide through sustainable community development projects, and preparing future leaders who are well ...
Weight, Willis +1 more
core
FEBS Open Bio, EarlyView.Using peripheral blood for determining B‐cell or T‐cell clonality is more reliable when we use cell‐free RNA (cfRNA) because cells release blood significantly more RNA than DNA. Next‐generation sequencing (NGS) of cfRNA allows us to evaluate fragment cfRNA and evaluate clonality reliably without the need for prior determination of the specific dominant
Adam Albitar +11 more
wiley +1 more source
Engineers Without Borders - Guatemala - 2013 - 214
, 2019 The Carroll College Chapter of Engineers Without Borders USA is dedicated to improving the quality of life for people in developing communities worldwide through sustainable community development projects, and preparing future leaders who are well ...
Weight, Willis +1 more
core
Comparative assessment of crystallographic and cryo‐EM models in the Protein Data Bank
FEBS Open Bio, EarlyView.Raw data obtained by X‐ray crystallography or cryo‐EM result in experimental maps, ultimately fitted by atomic models. Although the physical principles are different, the final results can be viewed, compared, and evaluated in the same way. With cryogenic electron microscopy (cryo‐EM) on track to surpass X‐ray crystallography as the preferred method ...
Alexander Wlodawer +7 more
wiley +1 more source
Engineers Without Borders - Guatemala - 2019 - 159
, 2019 The Carroll College Chapter of Engineers Without Borders USA is dedicated to improving the quality of life for people in developing communities worldwide through sustainable community development projects, and preparing future leaders who are well ...
Weight, Willis +1 more
core
FEBS Open Bio, EarlyView.In this study, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum was biochemically and structurally characterized. Our results demonstrate that Ta0887 is a highly thermostable esterase that preferentially hydrolyzes p‐nitrophenyl hexanoate and possesses an α‐helical cap domain that likely contributes to its substrate ...
Alejandro Delgado‐Rey +4 more
wiley +1 more source