A proteomics of gills approach to understanding salinity adaptation of Scylla paramamosain
Gene, 2018Scylla paramamosain (Crustacea) is a commercially important euryhaline species distributed along the coast of southern China and other Indo-Pacific countries. However, a sudden variation in salinity will cause injury or even death to S. paramamosain.
Huan Wang +5 more
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SpToll1 and SpToll2 modulate the expression of antimicrobial peptides in Scylla paramamosain
Developmental & Comparative Immunology, 2018Tolls and Toll-like receptors (TLRs) were the first pattern recognition receptors (PRRs) identified to play key roles in host innate immunity. However, relatively little is known about other types of Toll-like receptors in Scylla paramamosain, although a Toll-like receptor (SpToll1) has recently been cloned.
Yan, Chen +8 more
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Characterization and expression profile of Vitellogenin gene from Scylla paramamosain
Gene, 2013The full-length (7816 bp) cDNA of Vitellogenin (Vtg) encoding 2560 aa with an estimated molecular mass of 287.743 kDa was cloned from the green mud crab Scylla paramamosain. Semi-quantitative PCR (sq-PCR) revealed a specific expression pattern of Sp-vtg gene in ovaries and hepatopancreas.
Xiwei, Jia +5 more
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Immunomodulatory role of short neuropeptide F in the mud crab Scylla paramamosain
Developmental & Comparative Immunology, 2022Short neuropeptide F (sNPF) is bioactive peptide secreted by neurons of invertebrates. It is one of the important pleiotropic neural molecules that is associated with a variety of physiological processes in invertebrates. However, little is known about the role of sNPF in the immune response. This study aimed to determine the distribution, localization,
Zhanning, Xu +4 more
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Mapping and characterization of antigenic epitopes of arginine kinase of Scylla paramamosain
Molecular Immunology, 2015Arginine kinase (AK) is a panallergen present in crustaceans, which can induce an immunoglobulin (Ig) E-mediated immune response in humans. The aim of this work was to map and characterize the antigenic epitopes of Scylla paramamosain AK. Specific-protein-A-enriched IgG raised in rabbits against purified S.
Yang, Yang +6 more
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Hepatopancreas cell cultures from mud crab, Scylla paramamosain
In Vitro Cellular & Developmental Biology - Animal, 2009Hepatopancreas is an important digestive and endocrine organ in crustacean. However, there are few reports on cell cultures from crabs. Here, the cell cultures of hepatopancreas from Scylla paramamosain was studied in vitro. Both the primary cell culture and subculture were grown in Leibovitz' L-15 medium, M199 medium, or a specially designed medium ...
Zeng, Hui +4 more
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Identification and functional analysis of the doublesex gene in the mud crab Scylla paramamosain
Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 2022Doublesex (Dsx) is a crucial member of the Dmrt gene family and plays a vital role in sex determination and differentiation among the animal kingdom. In the present study, a doublesex (designated as Spdsx) gene was identified and characterized for the first time in the mud crab, Scylla paramamosain. The Spdsx cDNA contains an 801 bp open reading frame (
Haifu, Wan +4 more
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Identification and characterization of a novel galectin from the mud crab Scylla paramamosain
Fish & Shellfish Immunology, 2020Galectins are a family of β-galactoside-binding lectins that play key roles in the invertebrate innate immunity system, but no galectin genes have been identified in the mud crab (Scylla paramamosain) so far. The present study is the first to clone a galectin gene (SpGal) from S.
Zhouyi Zhang +9 more
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Osmoregulation and Na,K-ATPase expression in osmoregulatory organs of Scylla paramamosain
Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 2006Scylla paramamosain is a euryhaline marine crab that can actively regulate its osmotic pressure of the hemolymph. We investigated the expression of Na,K-ATPase in the osmoregulatory organs, including anterior gills, posterior gills, antennal glands, and midgut. Our cDNA sequencing of the Na,K-ATPase alpha-subunit revealed no isoforms in any of the four
Kuei-Fang, Chung, Hui-Chen, Lin
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Scylla paramamosain Transcriptome or Gene expression
2014The transcriptome and the expression profiling under the infection provides an invaluable new data for a functional genomics resource and biological research in S.paramamosain. The signaling cascades are regulated by V.parahaemolyticus infection in mud crab. These results will facilitate our comprehensive understanding of the mechanisms involved in the
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