Results 171 to 180 of about 3,091 (216)
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Molecular Biology Reports, 2020
Tropomyosin, a muscle tissue protein is a major allergen in most of shellfish including mud crab. Quantitative real time-PCR (qRT-PCR) using a stable reference gene is the most sensitive approach to produce accurate relative gene expression that has yet to be demonstrated for allergenic tropomyosin in mud crab species.
Nur Farah Hani Azemi +3 more
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Tropomyosin, a muscle tissue protein is a major allergen in most of shellfish including mud crab. Quantitative real time-PCR (qRT-PCR) using a stable reference gene is the most sensitive approach to produce accurate relative gene expression that has yet to be demonstrated for allergenic tropomyosin in mud crab species.
Nur Farah Hani Azemi +3 more
openaire +2 more sources
Environmental Pollution, 2021
Polychlorinated biphenyls (PCBs) are persistent organic pollutants in environments, and they can negatively affect aquatic animal health. After 7 days of PCBs exposure, the activities of catalase, phenoloxidase, and superoxide dismutase and the total hemocyte count in the haemolymph were significantly decreased and the reactive oxygen species (ROS ...
Chongyang, Xiao, Yunfei, Zhang, Fei, Zhu
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Polychlorinated biphenyls (PCBs) are persistent organic pollutants in environments, and they can negatively affect aquatic animal health. After 7 days of PCBs exposure, the activities of catalase, phenoloxidase, and superoxide dismutase and the total hemocyte count in the haemolymph were significantly decreased and the reactive oxygen species (ROS ...
Chongyang, Xiao, Yunfei, Zhang, Fei, Zhu
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Immunomodulatory role of short neuropeptide F in the mud crab Scylla paramamosain
Developmental & Comparative Immunology, 2022Short neuropeptide F (sNPF) is bioactive peptide secreted by neurons of invertebrates. It is one of the important pleiotropic neural molecules that is associated with a variety of physiological processes in invertebrates. However, little is known about the role of sNPF in the immune response. This study aimed to determine the distribution, localization,
Zhanning, Xu +4 more
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Hepatopancreas cell cultures from mud crab, Scylla paramamosain
In Vitro Cellular & Developmental Biology - Animal, 2009Hepatopancreas is an important digestive and endocrine organ in crustacean. However, there are few reports on cell cultures from crabs. Here, the cell cultures of hepatopancreas from Scylla paramamosain was studied in vitro. Both the primary cell culture and subculture were grown in Leibovitz' L-15 medium, M199 medium, or a specially designed medium ...
Zeng, Hui +4 more
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Dietary protein requirement of juvenile mud crab Scylla paramamosain
Aquaculture, 2020Abstract This experiment was carried out to investigate the optimal dietary protein levels for juvenile Scylla paramamosain. Six isoenergtic and isolipidic semi-purified diets were formulated with graded protein levels, and the final protein concentrations were 32.69%, 38.54%, 42.92%, 49.89%, 52.09% and 60.07%, respectively.
Puqiang Zheng +7 more
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SUMO-1 of mud crab (Scylla paramamosain) in gametogenesis
Gene, 2012The small ubiquitin-related modifier-1 (SUMO-1) is a member of a family of ubiquitin-related proteins. SUMO pathway, which is involved in gene expression in eukaryotic posttranslational processing, plays important roles in gene expression, genomic stability and the occurrence of cells, development and other biological processes.
Yanbin, Dai +5 more
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Characterization of gonad differentially expressed SoxB2 genes in mud crab Scylla paramamosain
Gene, 2020Members of sox gene family play critical roles in development, and some of them have crucial functions in sexual dimorphism. To understand the role of two SoxB2 genes, Sox14b and Sox21 of mud crab Scylla paramamosain, the full-length 1939 bp SpSox14b cDNA sequence and 861 bp SpSox21 cDNA sequence were obtained from the crab's transcriptome database ...
Jiaqian, Liao +4 more
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Characterization and expression profile of Vitellogenin gene from Scylla paramamosain
Gene, 2013The full-length (7816 bp) cDNA of Vitellogenin (Vtg) encoding 2560 aa with an estimated molecular mass of 287.743 kDa was cloned from the green mud crab Scylla paramamosain. Semi-quantitative PCR (sq-PCR) revealed a specific expression pattern of Sp-vtg gene in ovaries and hepatopancreas.
Xiwei, Jia +5 more
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Immunomodulatory role of crustacean cardioactive peptide in the mud crab Scylla paramamosain
Fish & Shellfish Immunology, 2022Crustacean cardioactive peptide (CCAP) is a pleiotropic neuropeptide, but its immunomodulatory role is not clear. Herein, the mud crab Scylla paramamosain provides a primitive model to study crosstalk between the neuroendocrine and immune systems. In this study, in situ hybridization showed that Sp-CCAP positive signal localized in multiple cells in ...
Yujie, Wei +5 more
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Identification of physicochemical properties of Scylla paramamosain allergen, arginin kinase
Journal of the Science of Food and Agriculture, 2012AbstractBACKGROUND: Arginine kinase (AK) is expressed in a wide variety of species, including human food sources (seafood) and pests (cockroaches and moths), and has been reported as a novel allergen. However, there has been little research on the allergenicity of AK in crustaceans.
Hui-Lin, Yu +5 more
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