Results 21 to 30 of about 951,836 (308)

Seed protein electrophoresis for assessment of genetic variation within genotypes of meadow fescue (Festuca pratensis Huds)

Czech Journal of Genetics and Plant Breeding, 2010
The protein diversity of 45 genotypes (forty genotypes selected within two gene pools and five varieties) of meadow fescue was examined. Genetic variation was described using ISTA/UPOV methods for crop variety identification.
S. Stoyanova, B. Boller
doaj   +1 more source

PROFIL SDS-PAGE DAN AKTIVITAS LISOZIM PUTIH TELUR AYAM LOKAL TERMODIFIKASI PANAS

Jurnal Pangan dan Agroindustri, 2021
Lisozim adalah enzim hidrolase yang bisa diisolasi dari putih telur ayam. Penelitian bertujuan untuk mengarakterisasi lisozim termodifikasi dari putih telur ayam lokal dengan SDS-PAGE dan mengukur aktivitas lisozimnya.
Syahrizal Nasution, Didah Nur Faridah, Eni Kusumaningtyas, Zakiah Wulandari, Harsi Dewantari Kusumaningrum   +4 more
doaj   +1 more source

Salt-inducible kinases (SIKs) regulate TGFβ-mediated transcriptional and apoptotic responses [PDF]

, 2020
The signalling pathways initiated by members of the transforming growth factor-β (TGFβ) family of cytokines control many metazoan cellular processes, including proliferation and differentiation, epithelial-mesenchymal transition (EMT) and apoptosis. TGFβ
A Rojas-Fernandez, A Woods, AR Ramjaun, AY Hsing, B Bierie, B Ozdamar, B-C Kim, BR Binder, C Allan, CJ David, CS Hill, DB Shackelford, EC Connolly, FA Ran, G Avery-Cooper, G Rena, G Rosti, GD Cuny, GJ Inman, GJ Inman, GM Keating, H Ikushima, J Massagué, J Massagué, J Massaous, J Ozanne, J Song, J Vogt, J Xu, JF Callahan, JL Wrana, JM Lizcano, Joan Massagué, JY Altarejos, K Clark, KA Brown, KJ Livak, LC Spender, LC Spender, M Abe, M Kowanetz, M Tojo, MO Li, MR Keeton, NJ Bright, P Lönn, PH Lin, PK Datta, PM Siegel, RJ Akhurst, RJ Akhurst, S DaCosta Byfield, S Dennler, S Edlund, S Strickson, SA Hawley, T Sonntag, TB Sundberg, Y Drabsch, Y Liu, Y Shi, Y Yang, Y Zhang, Y Zhang   +63 more
core   +2 more sources

Molluscan Shell Matrix Characterization by Preparative SDS-PAGE

The Scientific World Journal, 2003
The glycoproteinaceous constituents of molluscan shell matrices usually resist chromatographical fractionation. We describe a protocol that overcomes this difficulty and permits collection of a large amount of shell proteins for further in vitro ...
Frederic Marin
doaj   +1 more source

SDS-PAGE v1

, 2021
SDS-PAGE gels are used to visualize proteins. This protocol describes how to prepare all the buffers required for casting and running SDS-PAGE gels, as well as how to prepare whole cell samples.
Anna Bird, Chiara Gandini
openaire   +1 more source

Comparative characterization of two GDP-mannose dehydrogenase genes from Saccharina japonica (Laminariales, Phaeophyceae) [PDF]

, 2016
SDS-PAGE analysis of recombinant SjGMDs.
Delin Duan, Pengyan Zhang, Weihua Jin, Zhanru Shao   +3 more
core   +2 more sources

Electrophoretic behavior of streptavidin complexed to a biotinylated probe : A functional screening assay for biotin-binding proteins [PDF]

, 2004
The biotin-binding protein streptavidin exhibits a high stability against thermal denaturation, especially when complexed to biotin. Herein we show that, in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), streptavidin is stabilized ...
Humbert, Nicolas, Ward, Thomas R., Zocchi, Andrea   +2 more
core   +2 more sources

Fluorescence-based proteasome activity profiling [PDF]

, 2012
With the proteasome emerging as a therapeutic target for cancer treatment, accurate tools for monitoring proteasome (inhibitor) activity are in demand.
Berkers, C.R., Jong, A., Ovaa, H., Rodenko, B., Schuurman, K.G.   +4 more
core   +1 more source

A FAST AND SENSITIVE ZYMOGRAPHY METHOD OF PEROXIDASE ACTIVITY DETERMINATION USING SODIUM ACETATE BUFFER

Studia Universitatis Babes-Bolyai Chemia, 2019
By changing the buffer composition from phosphate to acetate (in both cases, the pH value was 6.0), the sensitivity of the estimation of peroxidase activity in polyacrylamide gels was improved from 4 µg/well to 0.4 µg/well.
Gheorghița MENGHIU, Amalia Nicoleta IANCU, Vasile OSTAFE   +2 more
doaj   +1 more source

Detection of the basement membrane-degrading proteolytic activity of Paracoccidioides brasiliensis after SDS-PAGE using agarose overlays containing Abz-MKALTLQ-EDDnp [PDF]

, 1999
We have characterized, in the Paracoccidioides brasiliensis yeast phase, an exocellular SH-dependent serine proteinase activity against Abz-MKRLTL-EDDnp and analogous fluorescent-quenched peptides, and showed that it is also active against constituents ...
Carmona, Adriana Karaoglanovic, Juliano, Luiz, Juliano, Maria Aparecida, Puccia, Rosana, Travassos, Luiz Rodolpho   +4 more
core   +4 more sources