Results 21 to 30 of about 4,922,643 (264)
SDS-PAGE and Western blot analysis of fusion protein.
, 2021 (A)SDS-PAGE result of fusion protein in the process of purification (M, marker; lane1, loading solution; lane2, flow-through solution; lane3-4, 20 mM imidazole elution fraction; lane5, 50 mM imidazole elution fraction; lane6, 500 mM imidazole elution ...
Qiongqiong Bai (11285977) +7 more
core +1 more source
Czech Journal of Genetics and Plant Breeding, 2010 The protein diversity of 45 genotypes (forty genotypes selected within two gene pools and five varieties) of meadow fescue was examined. Genetic variation was described using ISTA/UPOV methods for crop variety identification.
S. Stoyanova, B. Boller
doaj +1 more source
SDS-PAGE-Western blot of different HIV-1 Env strains bearing I559P alone or in combination with NFL.
, 2023 (A) VLPs expressing Q23-17 (Lanes 1–6), T250-4 (Lanes 7–12) and WITO.33 (Lanes 13–18), parent and mutant combinations were lysed and boiled in SDS/DTT, followed by digestion with endo H or PBS.
Tommy Tong (426980) +7 more
core +1 more source
SDS-PAGE and mass spectrometry analyses.
, 2022 Bacterial lysates (50 μg) of the original (OG) and mutant (MT) strains were subjected to an SDS-PAGE analysis, followed by CBB staining. A major band (arrowhead), which is clearly detected in MT, was identified as the TDE_1072 protein by mass ...
Mari Fujita (397912) +4 more
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Molluscan Shell Matrix Characterization by Preparative SDS-PAGE
The Scientific World Journal, 2003 The glycoproteinaceous constituents of molluscan shell matrices usually resist chromatographical fractionation. We describe a protocol that overcomes this difficulty and permits collection of a large amount of shell proteins for further in vitro ...
Frederic Marin
doaj +1 more source
Fermentációs minták fehérjetartalmának az analízise SDS-PAGE módszerrel
, 2021 A vizsgálat célja az volt, hogy fermentációk lizozim termelését kövessük nyomon. A vizsgált fehérjét az Aspergillus niger fonalas gomba termelte. Mindkét esetben SDS-PAGE módszerrel követtük nyomon a lizozim mennyiségének a változását.hbkBiomérnökiBSc ...
Rab, Polett
core
SDS-PAGE of Val143 variants under reducing conditions.
, 2020 Coomassie Brilliant Blue-stained 12.5% SDS-polyacrylamide gel showing marker proteins (Protein Markers for SDS-PAGE, Nacalai Tesque) and purified enzyme preparations of WT and 17 Val143 variants. The original gels are shown in S4 Fig.
Takuya Sugiura (8463252) +7 more
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SDS-PAGE analysis of complement factors (cFactors) treated with PG0352.
, 2023 For this experiment, individual complement factors were treated with wild-type PG0352 or inactive PG0352 (PG0352AAAA) for 60 minutes and then subjected to 10% SDS-PAGE followed by Coomassie blue staining. FH: factor H; FI: factor I. (TIFF)
Kurni Kurniyati (9588578) +8 more
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FEBS Letters, EarlyView.Septin 9 polybasic domains couple phosphoinositide‐rich membrane binding to centrosome positioning, Golgi organization, and microtubule acetylation to control epithelial polarity. Their loss disrupts this axis, causing centrosome mispositioning, Golgi fragmentation, reduced microtubule acetylation, and polarity inversion via upregulation of the ...
Ting ting Cai +4 more
wiley +1 more source
Studia Universitatis Babes-Bolyai Chemia, 2019 By changing the buffer composition from phosphate to acetate (in both cases, the pH value was 6.0), the sensitivity of the estimation of peroxidase activity in polyacrylamide gels was improved from 4 µg/well to 0.4 µg/well.
Gheorghița MENGHIU +2 more
doaj +1 more source