Results 31 to 40 of about 4,922,643 (264)

USE OF MORPHOLOGICAL TRAITS AND ELECTRO- PHORESIS OF PROTEINS TO IDENTIFY THE TELEOMORPH OF Oidium lini INVOLVED IN POWDERY MILDEW OF FLAX [PDF]

open access: yesJournal of Plant Production, 2002
Powdery mildew (PM) of flax is caused by the obligate parasite Odium lini. One obvious gap in our current knowledge of O. lini is the lack of consensus regarding the name of its teleomorph, which has been referred to as Erysiphe polygoni or E ...
M. Mansour   +4 more
doaj   +1 more source

Degradation mechanism of the von Willebrand factor A2 domain by nattokinase

open access: yesFEBS Letters, EarlyView.
Nattokinase, a natto‐derived protease, exhibits potent antithrombotic effects. This study demonstrates that nattokinase directly cleaves the von Willebrand factor (vWF) A2 domain in vitro. Unlike the native regulator ADAMTS13, nattokinase degrades folded vWF independently of shear stress.
Ryuichi Hyakumoto   +3 more
wiley   +1 more source

SDS-PAGE separation.

open access: yes, 2017
Separation by SDS-PAGE of the digestion mixture of proteins after treatment with chymotrypsin. Proteins were digested at a mass ratio of protein to protease of 200:1 for 30 min at 30°C (left panel) and 20:1, overnight at 30°C (right panel).
José M. Delfino (736126)   +3 more
core   +1 more source

Salmonella lipopolysaccharide‐containing supported lipid bilayers as platforms to study bacteriophage interactions

open access: yesFEBS Letters, EarlyView.
We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace   +6 more
wiley   +1 more source

Comparative Analysis of Electrophoretic Profile of Major Proteins of Milk from Alpine and Carpathian Goats

open access: yesBulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Veterinary Medicine, 2017
The milk’s proteins provide nutritional and biologically active values, essential in human and animal nutrition. In the case of goat milk, the proteins’ concentration and quality represent basic indices for the evaluation of the nutritional and ...
Alina NASALEAN   +4 more
doaj   +1 more source

SDS-PAGE and Western blot analysis of recombinant IL-18.

open access: yes, 2016
(A) SDS-PAGE analysis of secreted proteins from the P. pastoris KM71 before purification. (B) SDS-PAGE analysis of recombinant IL-18 after purification with HisTrap affinity column, (C) Western blot analysis of recombinant IL-18 protein. All samples were
Varomyalin Tipmanee (2113162)   +5 more
core   +1 more source

Structural insights and therapeutic targets in Acinetobacter baumannii capsule biosynthesis

open access: yesFEBS Letters, EarlyView.
Hypervirulent KL49 A. baumannii's capsular polysaccharide contains the nonulosonic acid 8‐epi‐Leg5,7Ac2, synthesized by epimerization via ElaA, ElaB, and ElaC. Crystal structures of ElaA, ElaB, and ElaC reveal their role in CMP‐Leg5,7Ac2 synthesis and regioselective C8 epimerization.
Woo Cheol Lee   +7 more
wiley   +1 more source

SDS-PAGE analysis of the purified IgM and IgY.

open access: yes, 2016
The IgM and IgY containing fractions were concentrated using a 100 kDa cut off centrifugal filter (Millipore), and the protein composition determined by SDS-PAGE. The left panel shows proteins separated and visualised using Coomassie staining on a 5% SDS-
Olli Vapalahti (87344)   +4 more
core   +1 more source

ABL kinase‐dependent phosphorylation of SH proteins promotes their direct interaction with CRK family SH2 domains

open access: yesFEBS Letters, EarlyView.
CT10 regulator of kinase (CRK) and CRK‐Like (CRKL) are signaling adaptors driving cell adhesion, motility, differentiation, and proliferation. SH2‐domain containing (SH) proteins are enriched in YXXP motifs which when phosphorylated create preferred binding sites for CRK family SH2 domains.
Phoebe M. Cousens   +8 more
wiley   +1 more source

GLUTENIN LOCI VARIABILITY OF CROATIAN WHEAT GERMPLASM [PDF]

open access: yesPoljoprivreda, 2012
Glutenins loci were used for variability estimation in 50 varieties of hexaploid winter wheat originated from Croatian breeding centres. Polyacrylamide gel electrophoresis (PAGE) in presence of sodium dodecyl sulphate (SDS) was used for determination of ...
Ivana Rukavina   +4 more
doaj  

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