Results 31 to 40 of about 686,170 (207)
, 2021 SDS-PAGE gels are used to visualize proteins. This protocol describes how to prepare all the buffers required for casting and running SDS-PAGE gels, as well as how to prepare whole cell samples.
Anna Bird, Chiara Gandini
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Molluscan Shell Matrix Characterization by Preparative SDS-PAGE
The Scientific World Journal, 2003 The glycoproteinaceous constituents of molluscan shell matrices usually resist chromatographical fractionation. We describe a protocol that overcomes this difficulty and permits collection of a large amount of shell proteins for further in vitro ...
Frederic Marin
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USE OF MORPHOLOGICAL TRAITS AND ELECTRO- PHORESIS OF PROTEINS TO IDENTIFY THE TELEOMORPH OF Oidium lini INVOLVED IN POWDERY MILDEW OF FLAX [PDF]
Journal of Plant Production, 2002 Powdery mildew (PM) of flax is caused by the obligate parasite Odium lini. One obvious gap in our current knowledge of O. lini is the lack of consensus regarding the name of its teleomorph, which has been referred to as Erysiphe polygoni or E ...
M. Mansour +4 more
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Studia Universitatis Babes-Bolyai Chemia, 2019 By changing the buffer composition from phosphate to acetate (in both cases, the pH value was 6.0), the sensitivity of the estimation of peroxidase activity in polyacrylamide gels was improved from 4 µg/well to 0.4 µg/well.
Gheorghița MENGHIU +2 more
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, 2020 SDS-PAGE is an analytical technique used to separate proteins based on their molecular weight using electrophoresis. Peptides migrate faster due to less resistance from the gel matrix. The peptide used in this experiment is characterised by a short length (molecular weight is less than 3kDa), so we have used a very dense (20%) gel in order to get a ...
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Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Veterinary Medicine, 2017 The milk’s proteins provide nutritional and biologically active values, essential in human and animal nutrition. In the case of goat milk, the proteins’ concentration and quality represent basic indices for the evaluation of the nutritional and ...
Alina NASALEAN +4 more
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Speeding up SDS–PAGE: Theory and experiment
ELECTROPHORESIS, 2023 AbstractIn order to accelerate Sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE), here we propose an optimized version of the technique enabled by experimental tuning reinforced by theoretical description. In the resulting system, the gel buffer was diluted twofold and supplemented with glycine at a low concentration, whereas a ...
Maria K. Koshkina +5 more
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Estimation of Protease Enzyme Bioactivity Isolated from Bacterial Strains [PDF]
Records of Pharmaceutical & Biomedical SciencesFifteen bacterial strains were isolated from soil samples collected from Northern East region of the delta Egypt. Bacterial strains were screened for proteolytic activity on saline skim milk agar medium where the most potent protease producer strain was ...
Reham Elkased
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GLUTENIN LOCI VARIABILITY OF CROATIAN WHEAT GERMPLASM [PDF]
Poljoprivreda, 2012 Glutenins loci were used for variability estimation in 50 varieties of hexaploid winter wheat originated from Croatian breeding centres. Polyacrylamide gel electrophoresis (PAGE) in presence of sodium dodecyl sulphate (SDS) was used for determination of ...
Ivana Rukavina +4 more
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Journal of Genetic Engineering and Biotechnology, 2016 Somatic chromosome study from root tip cells using the squash technique of the cytological method and seed protein profile of 5 varieties of Lens culinaris (Lentil) through SDS–PAGE were investigated.
Tuhin Pal +3 more
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