Results 61 to 70 of about 4,922,643 (264)
FEBS Letters, EarlyView.Encapsulins are protein nanocompartments that play an important role in iron storage. In the Myxococcus xanthus encapsulin system, two cargo proteins called EncB and EncC contribute to iron mineralization. Here, we show that EncB and EncC generate iron‐containing minerals with distinct chemical compositions, suggesting that the composition of stored ...
Harry B. McDowell +2 more
wiley +1 more source
Boletín de la Sociedad Argentina de Botánica, 2007 Colletotrichum truncatum is the most common pathogen fungus associated with soybean anthracnose. Although the lignin-degrading enzyme laccase has been implicated in pathogenicity of a wide range of plant pathogenic fungi, its biological role in the ...
L. Levin +3 more
doaj
SDS-PAGE and Western blot analysis of expressed 16mer-peptides.
, 2019 (a, c) SDS-PAGE analysis of expressed 16mer-peptides. The numbers of P1-P56 indicate each 16mer-peptide in cell total proteins. The cell proteins of each r-clone were resolved by 12% SDS-PAGE gel electrophoresis and stained with Coomassie brilliant blue.
Yijie Li (653857) +11 more
core +1 more source
SDS-PAGE and Western blot analysis of expressed 8mer-peptides.
, 2022 (A, C, E and G) SDS-PAGE analysis of expressed 8mer-peptides. It indicates each 8mer peptide of P3, P8, P9, P10, P12, P15, P19, P23, P29, P38 and P54. The cell proteins of each recombinant clone were resolved by 12% SDS-PAGE and stained with Coomassie ...
Boyong Jiang (13155018) +8 more
core +1 more source
Structural and biochemical analysis of a B12 superbinder
FEBS Letters, EarlyView.BtuG proteins are vitamin B12 scavengers in Bacteroides thetaiotaomicron, a dominant human gut bacterium. We present crystal structures of three BtuG homologs bound to cobalamin and its precursor cobinamide, revealing picomolar binding affinities, among the highest known for any natural protein.
Jose M. Martinez Felices +3 more
wiley +1 more source
FEBS Letters, EarlyView.Mycobacterial 3‐methylcrotonyl‐CoA carboxylase uses a mobile biotin‐carrying domain to shuttle a carboxyl group between two catalytic sites, enabling carboxylation of 3‐methylcrotonyl‐CoA during leucine breakdown. Cryo‐electron microscopy captures the carrier at both sites and reveals an inward loop movement that may prevent futile rebinding to the ...
Ajit Yadav +2 more
wiley +1 more source
Journal of Crop Protection, 2016 This study was undertaken to find out the optimum physicochemical parameters of fermentation, i.e. pH, incubation temperature and incubation time for the cellulase enzyme production of Trichoderma harzianum. The extracellular protein content was estimated
Samira Shahbazi +2 more
doaj
Separating proteins using SDS Page
, 2011 An articulate presentation detailing the theory and practice of protein separation using SDS PAGE. This unit includes a video demonstration of how to set up and run an SDS PAGE gel.
Dr Sue Whittle, Dr Sue Bickerdike
core
SDS-PAGE analysis of the purified Fabs.
, 2019 Purified Fabs 2A6 and 4E2 were preheated and then individually resolved by SDS-PAGE under denaturing and reducing conditions. The heterodimer was dissociated into the light chain (28 kDa) and the Fd fragment (32 kDa). Lane M represents the protein marker.
Yuting Xian (6883502) +6 more
core +1 more source
The Shewanella oneidensis Fic enzyme SoFic targets the switch‐I region of EF‐Tu for AMPylation
FEBS Letters, EarlyView.Fic enzymes mediate diverse post‐translational modifications across all domains of life, including AMPylation. Prokaryotic EF‐Tu can be AMPylated and deAMPylated by the conserved Fic enzyme SoFic. Structural and biochemical approaches were used to characterize the effect of AMPylation on EF‐Tu, SoFic's enzymatic activities, and the enzyme‐target ...
Svenja Runge +6 more
wiley +1 more source