Results 51 to 60 of about 1,160,569 (281)

SDS-PAGE analysis of complement factors (cFactors) treated with PG0352.

open access: yes, 2023
For this experiment, individual complement factors were treated with wild-type PG0352 or inactive PG0352 (PG0352AAAA) for 60 minutes and then subjected to 10% SDS-PAGE followed by Coomassie blue staining. FH: factor H; FI: factor I. (TIFF)
Kurni Kurniyati (9588578)   +8 more
core   +1 more source

Serum Myonectin Levels Are Positively Associated With Physical Function and Lower Frailty‐Related Limitation in Maintenance Hemodialysis Patients: A Cross‐Sectional Study

open access: yesTherapeutic Apheresis and Dialysis, EarlyView.
ABSTRACT Background Maintenance hemodialysis (MHD) patients frequently suffer from frailty, characterized by reduced physical function and poor prognosis. Myokines, such as myonectin, secreted by muscle, are emerging regulators of systemic health. This study investigated the relationship between serum myonectin, adipokines (adiponectin, omentin), and ...
Kenichi Kono   +7 more
wiley   +1 more source

Association of TGF-β1 with periodontitis patients in north Indian population

open access: yesJournal of Oral Biology and Craniofacial Research
Introduction: Periodontitis is a complex disease caused by environmental and genetic factors. The course of periodontal destruction is influenced by local, ecological, and, predominantly, by genetic factors.
Ruchi Pandey   +5 more
doaj   +1 more source

Structural instability impairs function of the UDP‐xylose synthase 1 Ile181Asn variant associated with short‐stature genetic syndrome in humans

open access: yesFEBS Letters, EarlyView.
The Ile181Asn variant of human UDP‐xylose synthase (hUXS1), associated with a short‐stature genetic syndrome, has previously been reported as inactive. Our findings demonstrate that Ile181Asn‐hUXS1 retains catalytic activity similar to the wild‐type but exhibits reduced stability, a looser oligomeric state, and an increased tendency to precipitate ...
Tuo Li   +2 more
wiley   +1 more source

SDS-PAGE separation.

open access: yes, 2017
Separation by SDS-PAGE of the digestion mixture of proteins after treatment with chymotrypsin. Proteins were digested at a mass ratio of protein to protease of 200:1 for 30 min at 30°C (left panel) and 20:1, overnight at 30°C (right panel).
José M. Delfino (736126)   +3 more
core   +1 more source

The (Glg)ABCs of cyanobacteria: modelling of glycogen synthesis and functional divergence of glycogen synthases in Synechocystis sp. PCC 6803

open access: yesFEBS Letters, EarlyView.
We reconstituted Synechocystis glycogen synthesis in vitro from purified enzymes and showed that two GlgA isoenzymes produce glycogen with different architectures: GlgA1 yields denser, highly branched glycogen, whereas GlgA2 synthesizes longer, less‐branched chains.
Kenric Lee   +3 more
wiley   +1 more source

SDS-PAGE and Western blot analysis of recombinant IL-18.

open access: yes, 2016
(A) SDS-PAGE analysis of secreted proteins from the P. pastoris KM71 before purification. (B) SDS-PAGE analysis of recombinant IL-18 after purification with HisTrap affinity column, (C) Western blot analysis of recombinant IL-18 protein. All samples were
Varomyalin Tipmanee (2113162)   +5 more
core   +1 more source

فراوانی ژنpxo1 در باسیلوس سرئوس، باسیلوس تورنجینزیس و باسیلوس سوبتیلیس با استفاده از تکنیک SDS-PAGE [PDF]

open access: yesمجله دانشگاه علوم پزشکی سبزوار, 1970
زمینه و هدف: در بین باسیلوس ها، باسیلوس سرئوس، باسیلوس تورنجینزیس و باسیلوس آنتراسیس دارای اهمیت زیادی می باشند که از میان آن ها باسیلوس آنتراسیس عامل بیماری سیاه زخم می باشد.
محمدجواد نمازی   +6 more
doaj  

Organ‐specific redox imbalances in spinal muscular atrophy mice are partially rescued by SMN antisense oligonucleotides

open access: yesFEBS Letters, EarlyView.
We identified a systemic, progressive loss of protein S‐glutathionylation—detected by nonreducing western blotting—alongside dysregulation of glutathione‐cycle enzymes in both neuronal and peripheral tissues of Taiwanese SMA mice. These alterations were partially rescued by SMN antisense oligonucleotide therapy, revealing persistent redox imbalance as ...
Sofia Vrettou, Brunhilde Wirth
wiley   +1 more source

SDS-PAGE analysis of the purified IgM and IgY.

open access: yes, 2016
The IgM and IgY containing fractions were concentrated using a 100 kDa cut off centrifugal filter (Millipore), and the protein composition determined by SDS-PAGE. The left panel shows proteins separated and visualised using Coomassie staining on a 5% SDS-
Olli Vapalahti (87344)   +4 more
core   +1 more source

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