Results 41 to 50 of about 174,695 (299)

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Experimental Research on Performance of New Gas Drainage Borehole Sealing Material with High Fluidity

Advances in Materials Science and Engineering, 2021
To ensure the sealing effect of gas extraction borehole in underground coal mine and improve the concentration of gas extraction, a kind of high-flow sealing material which can be injected into the microcracks around borehole was developed. The ultrafine
Xin Guo, Sheng Xue, Chunshan Zheng, Yaobin Li   +3 more
doaj   +1 more source

Secure Cloud Storage with Client-Side Encryption Using a Trusted Execution Environment

, 2020
With the evolution of computer systems, the amount of sensitive data to be stored as well as the number of threats on these data grow up, making the data confidentiality increasingly important to computer users.
da Rocha, Marciano, Gorgonio, Kyller Costa, Pagno, Rodrigo Tomaz, Perkusich, Angelo, Valadares, Dalton Cézane Gomes, Will, Newton Carlos   +5 more
core   +1 more source

YlmG1 is localized exclusively to the chloroplast envelope membrane and is involved in preprotein translocation in Arabidopsis thaliana

FEBS Open Bio, EarlyView.
Cytosolically synthesized chloroplast preproteins are translocated across the outer and inner envelope membranes through translocons called TOC and TIC, respectively. In green algae and plants, the TIC core is composed of essential membrane proteins, Tic12, Tic20, and Tic214.
Mengyi Li, Xueyang Zhao, Masato Nakai
wiley   +1 more source

Analysis and Optimization of Influencing Factors on Sealing Performance of Subsea Wellhead Connector

Shiyou jixie, 2022
As a key equipment to connect high-pressure wellhead with subsea Christmas tree and prevent the leakage of high-temperature and high-pressure fluid in the well, the subsea connector is faced with extremely severe load.
Liu Shujie, Meng Wenbo, Huang Yi, Liu Hexing, Li Lei, Huang Liang, Zhao Suwen   +6 more
doaj  

Poppet valve tester [PDF]

, 1973
Tester investigates fundamental factors affecting cyclic life and sealing performance of valve seats and poppets. Tester provides for varying impact loading of poppet against seat and rate of cycling, and controls amount and type of relative motion ...
Tellier, G. F.
core   +1 more source

Hyperactive ice‐binding proteins stabilize cell membranes and improve resistance to dehydration stress in Caenorhabditis elegans

FEBS Open Bio, EarlyView.
TisIBP8, a fungal‐derived hyperactive ice‐binding protein, helps Caenorhabditis elegans survive dehydration. It localizes near cell membranes, reduces cell damage, and helps maintain membrane structure during drying. These results suggest that ice‐binding proteins can protect cells from dehydration stress as well as freezing stress.
Daiki Shimose, Kotaro Ozaki, Ryohei Kuriyama, Yuka Ikemoto, Kazuhiro Mio, Yuji C. Sasaki, Tatsuya Arai, Sakae Tsuda, Yoichi Shinkai, Masahiro Kuramochi   +9 more
wiley   +1 more source

A Systematic Comparison of Alpha‐Synuclein Seed Amplification Assays for Increasing Reproducibility

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Seed amplification assays (SAAs) enable ultrasensitive detection of misfolded α‐synuclein across biofluids and tissues. Yet, heterogeneity in protocols limits cross‐study comparability and clinical translation. Here, we review α‐synuclein SAA methods and their performance across various biological matrices.
Manuela Amaral‐do‐Nascimento, Daniela F. Santos, Tuane C. R. G. Vieira, Tiago F. Outeiro   +3 more
wiley   +1 more source

Comparing the Effect of Semi‐Immersive Virtual Reality, Computerized Cognitive Training, and Traditional Rehabilitation on Cognitive Function in Multiple Sclerosis: A Randomized Clinical Trial

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Background Cognitive impairment is a common non‐motor symptom in Multiple Sclerosis (MS), negatively affecting autonomy and Quality of Life (QoL). Innovative rehabilitation strategies, such as semi‐immersive virtual reality (VR) and computerized cognitive training (CCT), may offer advantages over traditional cognitive rehabilitation (TCR ...
Maria Grazia Maggio, Desiree Latella, Lilla Bonanno, Paulo Cezar Rocha dos Santos, Marcos Maldonado‐Díaz, Meir Plotnik, Rosaria De Luca, Angelo Quartarone, Rocco Salvatore Calabrò   +8 more
wiley   +1 more source

Optimization of K-Shaped Metal Sealing Ring for Subsea Tubing Hanger Based on Response Surface Method

Modelling and Simulation in Engineering
The K-shaped metal sealing ring of the subsea tubing hanger is a crucial sealing component ensuring wellhead sealing. Optimizing its structure can enhance the sealing performance of the tubing hanger and improve the reliability of subsea wellhead ...
Huan Xie, Hongxia Zhang, Yiwei Fan, Liyuan Liu, Wei Zeng   +4 more
doaj   +1 more source