Results 161 to 170 of about 501,124 (211)

Reassigning an amber codon to selenocysteine using a functional cysteine suppression pair in <i>Escherichia coli</i>. [PDF]

Synth Syst Biotechnol
Niu S, Wei J, Li M, Guo B, Yan Q, Huang Y, Jiang Z, Song J.   +7 more
europepmc   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Hyphenated Mass Spectrometry-Based Strategies for Characterizing Oligomers of α‑Synuclein in Parkinson's Disease. [PDF]

ACS Cent Sci
Sadighi R, Istrati A, Karijodikoro S, Scheefhals G, Baerenfaenger M, Rijs AM.   +5 more
europepmc   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Exploring the Potential for Smartphone-Based Reflection to Support Socioemotional Competence Development in Student Nurses' Clinical Placement: Mixed Methods Field Trial. [PDF]

JMIR Nurs
Bril-Broers I, Degens N, Fleer J.
europepmc   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Reconstitution of glycan-driven MHC-I recycling reveals calreticulin as mediator between TAPBPR and tapasin. [PDF]

EMBO J
Heinke TJ, Fahim A, Popovic N, Rath T, Müller IK, Morgner N, Trowitzsch S, Tampé R.   +7 more
europepmc   +1 more source

Small RNA pathways in mammalian oocytes

FEBS Open Bio, EarlyView.
Three distinct small RNA pathways operate in mammalian oocytes: RNAi interference (RNAi), the microRNA (miRNA) pathway, and the PIWI‐associated RNA (piRNA) pathway. These pathways use small RNAs to guide sequence‐specific repression and contribute to oocyte biology by targeting genes and mobile elements or appear insignificant since different ...
Petr Svoboda, Josef Pasulka
wiley   +1 more source

Multiheme selenoenzyme essential for elemental sulfur respiration. [PDF]

Sci Adv
Mihara H, Yoshizawa T, Izu Y, Zhang W, Inoue M, Shimamoto N, Tobe R, Aono R, Kurihara T, Matsumura H.   +9 more
europepmc   +1 more source