Results 171 to 180 of about 2,518,839 (195)

Product of a New Gene, syd, Functionally Interacts with SecY when Overproduced in Escherichia coli [PDF]

Journal of Biological Chemistry, 1995
A mutant form of SecY, SecY-d1, was previously suggested to sequester a component(s) of the protein translocator complex. Its synthesis from a plasmid leads to interference with protein export in Escherichia coli. SecE is a target of this sequestration, and its overproduction cancels the export interference. We now report that overexpression of another
Akio Kihara, Tohru Yoshihisa, Koreaki Itô   +2 more
exaly   +3 more sources

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Presence of a gene in the archaebacterium Methanococcus vannielii homologous to secY of eubacteria

Biochimie, 1991
The nucleotide sequence of a gene located at the promoter-distal side of the 'spectinomycin-operon' homologue of the archaebacterium Methanococcus vannielii was determined. Its derived amino acid sequence displayed 20% (identical positions) or 52% (including conservative exchanges) similarity, respectively, to SECY from E coli.
J, Auer, G, Spicker, A, Böck
exaly   +3 more sources

Yeast Gene Which Suppresses the Defect in Protein Export of a secY Mutant of E.coli1

The Journal of Biochemistry, 1991
To find factors participating in protein translocation in yeast, we screened a yeast genomic library for genes which, when introduced into Escherichia coli, suppressed secY24, a temperature sensitive mutation of an essential integral membrane protein (SecY) required for protein export.
Masao Sakaguchi, Chiharu Ueguchi, Koreaki Ito, Tsuneo Omura   +3 more
openaire   +2 more sources

Identification of the secY (prlA) gene product involved in protein export in Escherichia coli

Molecular and General Genetics MGG, 1984
The gene secY (or prlA) is essential for protein export across the cytoplasmic membrane of Escherichia coli. The protein product of secY has been identified using the gene cloned under the control of the pL promoter of phage lambda in combination with the maxicell system. The protein was found to have some unusual properties. First, it is important not
openaire   +2 more sources

The genes for ribosomal protein L15 and the protein equivalent to secY in the archaebacterium Haloarcula (Halobacterium) marismortui

Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1992
The nucleotide sequences of the L15 gene and the secY gene, which form the last two genes of the S10/spc-operon region of Haloarcula marismortui, have been determined. The HmaL15 protein sequence translated from the DNA is 164 amino acids long, revealing 10 amino acids more at the C-terminus than the published protein sequence.
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Flavescence dorée' phytoplasma strain differentiation in the translocase (secY) gene

2011
'Flavescence dorée' (FD) associated phytoplasmas are molecularly distinguished in strains belonging to 16SrV-C and -D subgroups that are geographically separated in the majority of the European countries where the disease was reported. While the subgroup differentiation on the 16S ribosomal gene is quite stable, RFLP analyses on the translocase gene ...
PALTRINIERI, SAMANTA, CONTALDO, NICOLETTA, F. Dal Molin, J. Mitrovic, DUDUK, BOJAN, BERTACCINI, ASSUNTA   +5 more
openaire   +3 more sources

Cloning and characterization of a Bacillus subtilis gene homologous to E. coli secY.

Journal of biochemistry, 1990
A 3.5-kb HindIII DNA fragment containing the secY gene of Bacillus subtilis has been cloned into plasmid pUC13 using the Escherichia coli secY gene as a probe. The complete nucleotide sequence of the cloned DNA indicated that it contained five open reading frames, and their order in the region, given by the gene product, was suggested to be L30-L15 ...
K, Nakamura, A, Nakamura, H, Takamatsu, H, Yoshikawa, K, Yamane   +4 more
openaire   +1 more source

Dual RNA-seq uncovers the immune response of Larimichthys crocea to the secY gene of Pseudomonas plecoglossicida from the perspective of host-pathogen interactions

Fish & Shellfish Immunology, 2019
Pseudomonas plecoglossicida is a Gram-negative aerobic bacterium that causes high mortality and serious economic losses in some commercial marine fish. Expression of secY was found to be significantly upregulated at 18 °C compared to 28 °C by RNA-seq and qRT-PCR.
Luying Wang, Yunjia Sun, Lingmin Zhao, Xiaojin Xu, Lixing Huang, Yingxue Qin, Yongquan Su, Jiaonan Zhang, Qingpi Yan   +8 more
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Molecular variability of secY gene in isolates of stolbur phytoplasma infected grapevine from Croatia

2010
Phytoplasmas are intracellular prokaryotic parasites/symbionts of the plant phloem and insect hemolymph. Grapevine yellows (GY) is economically important disease that affects grapevine (Vitis vinifera L.). Most important causative agents of GY in Europe are flavescence dorée (16SrV) and stolbur (bois noir, 16SrXII-A) phytoplasma.
Šeruga Musić, Martina, Pušić, Petra
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High-level expression in Escherichia coli of selenocysteine-containing rat thioredoxin reductase utilizing gene fusions with engineered bacterial-type SECIS elements and co-expression with the selA, selB and selC genes.

Journal of molecular biology, 1999
Mammalian thioredoxin reductase (TrxR) catalyzes reduction of thioredoxin and many other substrates, and is a central enzyme for cell proliferation and thiol redox control. The enzyme is a selenoprotein and can therefore, like all other mammalian selenoproteins, not be directly expressed in Escherichia coli, since selenocysteine-containing proteins are
E S, Arnér, H, Sarioglu, F, Lottspeich, A, Holmgren, A, Böck   +4 more
openaire   +1 more source