Results 241 to 250 of about 1,164,710 (315)

PEStimate: predicting offspring disease risk after polygenic embryo screening. [PDF]

Bioinformatics
Klausner L, Revital A, Lencz T, Carmi S.
europepmc   +1 more source

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

Genome association of carbohydrate metabolites provides new insights toward functional breeding in coffee. [PDF]

Plant Genome
Tavares Flores E, Spicer RL, Souza EMR, Ferrão MAG, Senra JFB, Azevedo C, Munoz PR, Tieman D, Voiniciuc C, Ferrão LFV.   +9 more
europepmc   +1 more source

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Putative Genomic Signatures of Local Adaptation in Five Local Indonesian Sheep Reveal Selection on Immunity, Reproduction, and Production Traits. [PDF]

Ecol Evol
Astuti PK, Mizeranschi AE, Herdis H, Sitaresmi PI, Ibrahim A, Prastowo S, Widyas N, Nugroho T, Kusza S.   +8 more
europepmc   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Genetic Diversity of the Beta-Casein Gene in Brown Swiss and Creole Cattle From the Peruvian Highlands. [PDF]

Anim Genet
Remigio Lorenzo LR, Rivera Romero C, De Souza Pacheco J, Bueno Cabrera W, Fuentes Navarro EL, Pichler R, Periasamy K, Gutiérrez-Reynoso GA.   +7 more
europepmc   +1 more source

Nuclear pore links Fob1‐dependent rDNA damage relocation to lifespan control

FEBS Open Bio, EarlyView.
Damaged rDNA accumulates at a specific perinuclear interface that couples nucleolar escape with nuclear envelope association. Nuclear pores at this site help inhibit Fob1‐induced rDNA instability. This spatial organization of damage handling supports a functional link between nuclear architecture, rDNA stability, and replicative lifespan in yeast.
Yamato Okada, Mina Iwaki, Kyosuke Hagiri, Rei Izumi, Masahiko Harata, Chihiro Horigome   +5 more
wiley   +1 more source

C2α‐carbanion‐protonating glutamate discloses tradeoffs between substrate accommodation and reaction rate in actinobacterial 2‐hydroxyacyl‐CoA lyase

FEBS Open Bio, EarlyView.
Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn, Barbara Seroka, Ryszard Lazny, Zenon Lotowski, Thore Rohwerder   +4 more
wiley   +1 more source

Estimation of Genetic Parameters of Body Weight and Body Size in Different Stages of Pishan Red Sheep. [PDF]

Animals (Basel)
Wujiwaili N, Huojiaabudula Y, Sataer B, Rouzimaimaiti A, Gong G, Qi A, Sulaiman Y.   +6 more
europepmc   +1 more source