Results 201 to 210 of about 1,462,497 (283)

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Sensitivity and specificity of immunohistochemistry for the diagnosis of filamentous fungal infections. [PDF]

Am J Clin Pathol
Thomas VL, Laga AC, Solomon IH.
europepmc   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Diagnostic Sensitivity and Specificity of Synovial Biomarkers in Knee Periprosthetic Infections. [PDF]

Cureus
Abaci YH, Yilmaz C.
europepmc   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Impact of Processing Delays and Cryopreservation on Sensitivity and Specificity of the Lymphocyte Transformation Test.

Int Arch Allergy Immunol
Heubach CA, Wurpts G, Glässner A, Grewe L, Dubrall D, Deck P, Weindl G, Hoffmann P, Nöthen M, Yazdi A, Sachs B.   +10 more
europepmc   +1 more source

Do Labial Minor Salivary Gland Biopsies Maintain Diagnostic Sensitivity and Specificity for Sjögren's Disease - An Institutional Study. [PDF]

Int J Appl Basic Med Res
Subramani V, Narasimhan M, Rajan ST, Harikrishnan T.   +3 more
europepmc   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Sensitivity and specificity of Dried Blood Spot and Plasma Separation Card samples for Hepatitis C Virus RNA Testing. [PDF]

PLOS Glob Public Health
Malobela A, Amougou-Atsama M, Iliopoulos P, Mugisha JC, Berishvili N, Sologashvili M, Fajardo E, Lamoury F, Macé A, Chirehwa M, Njouom R, Hatzakis A, Kabahizi J, Muvunyi CM, Buxton P, Mohiuddin S, Alkhazashvili M, Reipold EI.   +17 more
europepmc   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source