Results 231 to 240 of about 2,163,703 (333)

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

A noninferiority trial comparing the sensitivity and specificity of <i>Tritrichomonas foetus</i> RT-rtPCR using 0.9% sterile saline versus PBS as transport medium. [PDF]

J Vet Diagn Invest
Jumper TM, Thoresen M, King EH, Loy DS, Loy JD, Smith DR.   +5 more
europepmc   +1 more source

Measuring sleep: accuracy, sensitivity, and specificity of wrist actigraphy compared to polysomnography.

Sleep, 2013
Miguel Marino, Miguel Marino, Yi Li, M. Rueschman, J. Winkelman, J. Ellenbogen, J. Solet, H. Dulin, L. Berkman, O. Buxton   +9 more
semanticscholar   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Artificial intelligence-based diagnosis of non-displaced femoral neck fractures shows excellent sensitivity and specificity and reduces the need for computed tomography scans in emergency rooms. [PDF]

J Exp Orthop
Salzmann M, Diallo MR, Prill R, Becker R, Schreyer AG, Ostojic M, Ramadanov N.   +6 more
europepmc   +1 more source

Decreased cold‐sensing function of the transient receptor potential channel TRPM8 from tailed amphibians

FEBS Open Bio, EarlyView.
Despite frogs avoiding low temperatures, examination of four salamander species revealed that none avoided cold and all possessed cold tolerance. Functional analysis of TRPM8, a cold sensor, showed that all salamander TRPM8s had lost their cold sensitivity.
Tadahiro Sawao, Daiki Kobayashi, Shogo Hori, Osamu Saitoh   +3 more
wiley   +1 more source

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

MRI and MRA Sensitivity and Specificity for Accurate Diagnosis of Labral Tear for Patients Undergoing Hip Arthroscopy With Concomitant Periacetabular Osteotomy. [PDF]

Orthop J Sports Med
Zhang Y, Ellis T, Kollmorgen RC, Shkoukani Z, Martin RL.   +4 more
europepmc   +1 more source

Systemic dysregulation of apolipoproteins in amyotrophic lateral sclerosis serum

FEBS Open Bio, EarlyView.
Amyotrophic lateral sclerosis (ALS) is a fatal disease that damages motor neurons. This study found that people with ALS show significant changes in blood fats and the proteins that carry them. Several apolipoproteins were higher, lipid balances were altered, and normal protein–lipid relationships were disrupted.
Finula I. Isik, Russell Pickford, Hannah C. Timmins, Olivier Piguet, Glenda M. Halliday, Matthew C. Kiernan, Woojin Scott Kim   +6 more
wiley   +1 more source