Evolution‐guided yeast complementation reveals functional differences in human PSPH variants
Ancient genomes can help guide which human genetic variants are tested experimentally. This study applies that idea to PSPH, a gene involved in serine biosynthesis, and uses high‐throughput yeast complementation to compare variant function. The findings reveal measurable differences among selected alleles and illustrate the value of evolution‐guided ...
Mauricio Campa‐Álvarez +6 more
wiley +1 more source
Fatty-Acid Composition and Sensory Traits of Japanese Local Chickens in the Context of Asian Local Chicken Meat Quality. [PDF]
Takahashi H.
europepmc +1 more source
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
Frequency and Circumstances of Falls Events in People Living With Spinal and Bulbar Muscular Atrophy: A Cross-Sectional Survey. [PDF]
Lee LE +5 more
europepmc +1 more source
Dual native G‐quadruplex folding is associated with chromatin looping at the MYC locus
BG4‐detectable G‐quadruplex (G4) in HaCaT and NHEK keratinocytes identified folded and unfolded G4s enriched at promoters/TSSs and active enhancers, whereas unfolded G4s also overlapped weak/poised enhancers. At MYC–PVT1, 3C‐qPCR detected enhancer–promoter looping only when G4s were simultaneously folded at both regulatory elements under native ...
Dieila Giomo de Lima +7 more
wiley +1 more source
The art and science of head nerve blocks in urgent care settings. [PDF]
Rushetsky ZM +4 more
europepmc +1 more source
Gastrointestinal Microbiota Imbalance and Sensory Processing Dysregulation in Autism Spectrum Disorder: A Systematic Review. [PDF]
Bonner-Reid FT.
europepmc +1 more source
Longitudinal course of sensory processing difficulties in pediatric obsessive-compulsive disorder: A preliminary naturalistic observational study. [PDF]
Yamane K +6 more
europepmc +1 more source
Feeling the weight of post-COVID fatigue. [PDF]
Soteropoulos DS, Baker MR.
europepmc +1 more source

