Results 111 to 120 of about 39,003 (229)

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Sensory Difficulty and Assistive Device Use Among Older U.S. Immigrants: Differences by Region of Origin. [PDF]

Res Aging
Burns SD, Baker EH, Sheehan CM, Markides KS.   +3 more
europepmc   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Interventions to improve hearing aid use in adult auditory rehabilitation: A protocol for an updated systematic review and meta-analysis. [PDF]

PLoS One
Calvert S, Broome EE, Pourhoseingholi MA, Schoones JW, Xia J, Sereda M, Bateman P, MacKeith S, Chen S, Henshaw H.   +9 more
europepmc   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

CLINICAL CHARACTERISTICS OF DEBUT AND RISK FACTORS OF PEDIATRIC MULTIPLE SCLEROSIS

Мать и дитя в Кузбассе
The aim of the research – to study the features of the clinical picture, MRI data of the brain and spinal cord, to identify predisposing factors for the onset of multiple sclerosis (MS) in children and adolescents. Materials and methods.
Валерий Иванович Ларькин, Игорь Иванович Ларькин, Татьяна Аркадьевна Морозова, Степан Евгеньевич Аполлонов   +3 more
doaj  

Falls in Functional Neurological Disorder: Prevalence, Risk Factors and Clinical Implications. [PDF]

Eur J Neurol
Mohammadi Z, Keyvanfar A, Higgins R, Stone J, Coebergh J, Edwards MJ, Nielsen G.   +6 more
europepmc   +1 more source

Suppression of lung adenocarcinoma migration through organelle alkalization by human lactoferrin – albumin fusion

FEBS Open Bio, EarlyView.
This paper reveals how human lactoferrin–albumin fusion (hLF‐HSA) potently suppresses lung adenocarcinoma cell migration. hLF‐HSA upregulates NHE7, leading to Golgi alkalization, disruption of the Golgi secretome, downregulation of MMP1, and reversal of EMT. These findings suggest a novel Golgi‐targeting strategy to suppress cancer cell migration.
Hana Nopia, Masahiro Kimura, Daisuke Kurimoto, Atsushi Sato   +3 more
wiley   +1 more source

Editorial: From molecules to function: the world of mesencephalic trigeminal nucleus neurons in health and disease. [PDF]

Front Cell Neurosci
Bae YC, Toyoda H, Kang Y.
europepmc   +1 more source