Results 181 to 190 of about 2,821,158 (289)

Increasing Oligonucleotide Sequencing Information and Throughput with Ion Mobility Spectrometry-Mass Spectrometry. [PDF]

J Am Soc Mass Spectrom
Ryan JP, Slysz GW, Rye P, Stow SM, Dodds JN, Sausen J, Baker ES.   +6 more
europepmc   +1 more source

Construction of hyperthermostable d‐allulose 3‐epimerase from Arthrobacter globiformis M30 using the sequence information from Arthrobacter psychrolactophilus

FEBS Open Bio, EarlyView.
d‐Allulose can be produced from d‐fructose by d‐allulose 3‐epimerase. Based on sequence homology information, we successfully engineered thermostable mutants with the protein engineering method. By integrating positive mutations, we constructed an enzyme that exhibits hyperthermostability without a loss in the activity.
Kensaku Shimada, Kouhei Ohtani, Pushpa Kiran Gullapalli, Kazuhiko Ishikawa   +3 more
wiley   +1 more source

The Structural Deciphering of the α3 Helix Within ZmHsfA2'S DNA-Binding Domain for the Recognition of Heat Shock Elements in Maize. [PDF]

Plants (Basel)
Wang Y, Ma Z, Li G, Meng X, Duan S, Liu Z, Zhao M, Guo X, Zhang H.   +8 more
europepmc   +1 more source

Current trends in single‐cell RNA sequencing applications in diabetes mellitus

FEBS Open Bio, EarlyView.
Single‐cell RNA sequencing is a powerful approach to decipher the cellular and molecular landscape at a single‐cell resolution. The rapid development of this technology has led to a wide range of applications, including the detection of cellular and molecular mechanisms and the identification and introduction of novel potential diagnostic and ...
Seyed Sajjad Zadian, Khodakaram Jahanbin, Shekoofeh Nikooei, Marzieh Rostaminejad, Arezoo Rahimi, Pedram Abdizadeh, Behnam Alipoor   +6 more
wiley   +1 more source

Enhanced security for medical images using a new 5D hyper chaotic map and deep learning based segmentation. [PDF]

Sci Rep
Subathra S, Thanikaiselvan V.
europepmc   +1 more source

Biophysical characterization and ion transport with cell‐based and proteoliposome reconstitution assays of invertebrate K+‐Cl− cotransporters

FEBS Open Bio, EarlyView.
The K+‐Cl− cotransporters (KCCs) facilitate the symport of ions across the plasma membrane. They participate in physiological processes including neuronal regulation. Here, we characterized KCCs from Drosophila and Hydra vulgaris. Comparative analyses of transporters provide insights into the mechanism of KCC ion transport, regulation, and evolution ...
Satoshi Fudo, Marina Verkhovskaya, Coralie Di Scala, Claudio Rivera, Tommi Kajander   +4 more
wiley   +1 more source

Predicting rare DNA conformations via dynamical graphical models: a case study of the B→A transition. [PDF]

Nucleic Acids Res
De Silva N, Perez A.
europepmc   +1 more source

An efficient strategy for producing RNA‐free Nucleocapsid protein of SARS‐CoV‐2 for biochemical and structural investigations

FEBS Open Bio, EarlyView.
Cleavable N‐terminal Thioredoxin fusion enabled soluble expression and purification of otherwise insoluble SARS‐CoV‐2 Nucleocapsid (N) protein. A four‐step purification strategy yielded highly homogeneous, RNA‐free N protein. Binding assays showed high RNA affinity (Kd ~ 28 nm). The study will facilitate high‐resolution structural studies of N protein,
Shweta Singh, Gagan D. Gupta
wiley   +1 more source

A Novel Model for DNA Sequence Similarity Analysis Based on Graph Theory

Evolutionary Bioinformatics, 2011
Xingqin Qi, Qin Wu, Yusen Zhang, Eddie Fuller, Cun-Quan Zhang   +4 more
doaj  

Sequence analysis of the human DNA flanking sites of human immunodeficiency virus type 1 integration

Journal of Virology, 1996
S. Stevens, J. Griffith
semanticscholar   +1 more source