Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
Neisseria meningitidis Serogroup Y Sequence Type 1466 and Urogenital Infections. [PDF]
van Hal SJ +8 more
europepmc +1 more source
The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong +3 more
wiley +1 more source
Genomic characterization and virulence of Streptococcus suis serotype 7 sequence type 373 of clonal complex 94. [PDF]
Hatrongjit R +10 more
europepmc +1 more source
Acute caffeine treatment protects the developing retina from ischemia‐induced cell death
Caffeine reduces cell death in the developing retina under ischemia (OGD). This effect does not involve BDNF upregulation or antioxidant pathways (NRF2/VEGF). Neuroprotection occurs mainly through adenosine A2A receptor antagonism, decreasing glutamate release and excitotoxicity, highlighting caffeine's potential as an acute neuroprotective agent in ...
Amanda Alves Nascimento +6 more
wiley +1 more source
Machine learning-based lineage prediction from antimicrobial susceptibility testing phenotypes for <i>Escherichia coli</i> sequence type 131 clade C surveillance across infection types. [PDF]
Ross TA +7 more
europepmc +1 more source
Multilocus sequence type analysis of coagulase-negative staphylococci from the neonatal intensive care unit of a teaching hospital in Ghana. [PDF]
Afeke I +10 more
europepmc +1 more source
Unveiling a new sequence type in high-risk clonal complexes of virulent carbapenem-resistant Pseudomonas aeruginosa carrying bla<sub>KPC-2,</sub> bla<sub>NDM</sub> and bla<sub>oxa-48</sub>. [PDF]
de Almeida VF +10 more
europepmc +1 more source
Molecular Characterization of Vancomycin-Resistant <i>Enterococcus</i> spp. from Clinical Samples and Identification of a Novel Sequence Type in Mexico. [PDF]
Atriano Briano RA +4 more
europepmc +1 more source

