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Multilocus Sequence Typing of Bacteria
Annual Review of Microbiology, 2006Multilocus sequence typing (MLST) was proposed in 1998 as a portable, universal, and definitive method for characterizing bacteria, using the human pathogen Neisseria meningitidis as an example. In addition to providing a standardized approach to data collection, by examining the nucleotide sequences of multiple loci encoding housekeeping genes, or ...
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Frequency offset refocused PISEMA-type sequences
Journal of Magnetic Resonance, 2005The popular PISEMA experiment is highly sensitive to the 1H chemical shift dispersion and the choice of the 1H carrier frequency. This is due to the off-resonance 1H irradiation in the FSLG-CP sequence employed during the dipolar evolution period. In the modified approach described in this work, the interfering frequency offset terms are suppressed. In
Sergey V, Dvinskikh, Dick, Sandström
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HLA‐B locus sequence‐based typing
Tissue Antigens, 1999We describe an approach for HLA‐B high‐resolution typing. A single locus‐specific amplification generates a 1‐kb fragment useful for direct sequencing. Four internal primers are necessary for exon 2 and 3 cycle‐sequencing in both directions. Fluorescent dye‐labelled nucleotides are incorporated during cycle‐sequencing and reaction products are analyzed
S, Pozzi, A, Longo, G B, Ferrara
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HLA class I sequence-based typing
Human Immunology, 1993HLA oligogenotyping has been used successfully to characterize most phenotypically undetectable variants of class II genes. Limitations inherent to the class I system have, however, complicated the application of this and other molecular approaches to HLA class I typing.
P, Santamaria +6 more
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Multilocus Sequence Typing of Staphylococcus epidermidis
2013Multilocus sequence typing (MLST) is a genotyping method that is well suited for studying the population genetics and evolution of Staphylococcus epidermidis. The central MLST database for S. epidermidis continues to grow, and new analysis methods for extracting historical information from MLST data continue to be developed.
Jonathan C, Thomas, D Ashley, Robinson
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Identification of New Sequence Types for Multilocus Sequence Typing of Pediococcus pentosaceus
Food Engineering Progress, 2015The genus Pediococcus belongs to the lactic acid bacteria and includes 15 species which are used in the food industry as both starter and probiotic cultures. The importance of Pediococcus spp. is due to their use as starter cultures in fermented meat as well as to their presence as the natural microbiota in vegetables. The availability
Sulhee Lee, Young-Seo Park
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Multilocus sequence typing – what is resolved?
Trends in Microbiology, 2004Nucleotide sequence-based methods for bacterial typing (multilocus sequence typing; MLST) allow rapid and global comparisons between results from different laboratories. Combining this advantage with the reduced cost of high throughput sequencing, increasing automation and the amenability of sequence data for evolutionary analysis, it seems inevitable ...
Jessica E, Cooper, Edward J, Feil
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Sequenase sequence profiles used for HLA‐DPB1 sequencing‐based typing
Tissue Antigens, 1996Abstract: Sequencing‐based HLA typing (SBT) is a PCR based high resolution HLA typing method in which polymorphic regions of the gene are sequenced and directly used for typing. Currently, for class II SBT, alleles are identified by comparison of the exon 2 sequence with their corresponding allele sequence library.
Rozemuller, E.H. +6 more
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HLA‐DQB1 sequencing‐based typing updated
Tissue Antigens, 2007AbstractThe use of direct sequencing as a typing strategy is well acknowledged. Direct sequencing identifies all sequence motifs including new polymorphisms in heterozygous sequences. The earlier protocols for human leukocyte antigen HLA‐DQB1 Sequencing‐Based Typing (SBT) frequently encounter preferential amplification of one of the alleles that can ...
A, van Dijk +3 more
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Multilocus Sequence Typing of Staphylococcus aureus
2019Multilocus sequence typing (MLST) has been successfully used to differentiate and trace the bacterial species and pathogens that cause outbreaks or epidemics of infectious diseases. MLST provides a powerful solution for molecular epidemiological characterization of bacterial strains, including Staphylococcus aureus, by using the sequences of the ...
Ying, Liu, Yinduo, Ji
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