Results 261 to 270 of about 6,337,317 (349)

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

First Taxonomically Curated DNA Barcode Reference Library for Polish Myriapods Fills an Important Knowledge Gap for Soil Invertebrates in Central Europe, Part I: Millipedes (Myriapoda: Diplopoda). [PDF]

Ecol Evol
Bienias J, Trębicki Ł, Karlik G, Grabowski M.   +3 more
europepmc   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Identification and evolutionary analysis of insect fibrillin with their closely related family proteins. [PDF]

PeerJ
Chen J, Zhou F, Sun X, Li Z, Sun S, Zhang Y, Wan L.   +6 more
europepmc   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Redesign of energetically frustrated regions rescues function in defective T4 clamp loaders. [PDF]

Mol Biol Evol
Nimkar S, Nguyen T, Karandur D, Subramanian S, O'Donnell ME, Kuriyan J.   +5 more
europepmc   +1 more source

Refinement of amino‐acid conformation vs. difference density maps in time‐resolved serial femtosecond crystallography data analysis

FEBS Open Bio, EarlyView.
The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong, Yuhei Hosokawa, Lars Oliver Essen, Manuel Maestre‐Reyna   +3 more
wiley   +1 more source

Emergence and spread of NA-I223V and NA-S247N double-mutant A(H1N1)pdm09 influenza viruses with reduced oseltamivir susceptibility in the Netherlands and beyond, 2023 to 2026. [PDF]

Euro Surveill
Felix Garza Z, Eggink D, Bagheri M, van den Brink S, Goderski G, Pronk M, Lexmond P, Hooiveld M, van Gageldonk-Lafeber R, Koel B, Fouchier R, Meijer A.   +11 more
europepmc   +1 more source

cDNA sequences of three sheep myeloid cathelicidins

, 1995
SCOCCHI M, BAGELLA, Luigi Marco, ZANETTI M. CDNA SEQUENCES OF THREE SHEEP MYELOID CATHELICIDINS   +2 more
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