Results 71 to 80 of about 89,599 (313)

Expression and purification of E140 protein antigen fragments of Plasmodium vivax and Plasmodium berghei for serological assays

FEBS Open Bio, EarlyView.
We provide a step‐by‐step guide for producing E140 antigen fragments from Plasmodium berghei (Pb1) and Plasmodium vivax (Pv1). Pb1/Pv1 are expressed in E. coli, solubilized by freeze–thawing, refolded by slow dilution, purified by affinity chromatography (IMAC), then concentrated and subjected to quality control.
Rodolfo Ferreira Marques, Edit Ábrahám, Hiromi Muramatsu, Daniel Youssef Bargieri, Norbert Pardi, Zoltán Lipinszki   +5 more
wiley   +1 more source

Type 1 diabetes recurrence after SARS‐CoV‐2 infection in a subject with pancreas transplantation

Endocrinology, Diabetes &Metabolism, Volume 6, Issue 1, January 2023., 2023
During COVID‐19 pandemic, several studies have demonstrated a strong link between SARS‐CoV2 infection and diabetes mellitus. Little is known about the effects of SARS‐CoV‐2 infection on glycaemic control in pancreas transplant patients. Abstract Background During COVID‐19 pandemic, several studies have demonstrated a strong link between SARS‐CoV‐2 ...
Valentina Popolla, Alessandro Rizzi, Linda Tartaglione, Alfredo Pontecorvi, Dario Pitocco   +4 more
wiley   +1 more source

Textual analysis of clinical notes on pathology request forms to determine sensitivity and specificity of Hepatitis B and C virus infection status [PDF]

arXiv, 2022
Background: It is not established whether clinical notes provided on pathology request forms are useful as decision support data when assessing Hepatitis B and C viral infection status. Objective: To determine sensitivity, specificity, and predictive value of clinical notes for identifying infection status of Hepatitis B and C.
arxiv  

Tus-Ter-lock immuno-PCR assays for the sensitive detection of tropomyosin-specific IgE antibodies [PDF]

Bioanalysis, 2014, Vol. 6, No. 4, Pages 465-476, 2016
Background: The increasing prevalence of food allergies requires development of specific and sensitive tests capable of identifying the allergen responsible for the disease. The development of serologic tests that can detect specific IgE antibodies to allergenic proteins would therefore be highly received.
arxiv   +1 more source

High‐Throughput Immunoassays for Cavin‐4 IgG: A Diagnostic Tool for Immune‐Mediated Rippling Muscle Disease

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACTCavin‐4 was identified as a potential autoantigen for immune‐mediated rippling muscle disease (iRMD). To validate this, we developed and tested various immunoassays, including a cell‐based assay (CBA), cavin‐4 recombinant protein ELISA, and multi‐peptide ELISA. Among 19 iRMD patients, all exhibited muscle rippling, and 13 had percussion‐induced
Reghann G. LaFrance‐Corey, Haidara Kherbek, Nimalan Harinesan, Margherita Milone, Naveen K. Paramasivan, Pallab Sarker, Andrew M. Knight, Carley Karsten, Surendra Dasari, Teerin Liewluck, William J. Litchy, Sean J. Pittock, John R. Mills, Divyanshu Dubey   +13 more
wiley   +1 more source

Invalid Forensic Science Testimony and Wrongful Convictions [PDF]

, 2009
This is the first study to explore the forensic science testimony by prosecution experts in the trials of innocent persons, all convicted of serious crimes, who were later exonerated by post-conviction DNA testing.
Garrett, Brandon L., Neufeld, Peter J.
core   +2 more sources

Syphilis serology in HIV-positive and HIV-negative Nigerians: The public health significance [PDF]

, 2006
Syphilis has acquired new potential for morbidity and mortality through association with increased risk for HIV infection. Case-control survey was conducted using Rapid Plasma Reagin test and confirmatory Immunochromatographic test among HIV-positive ...
Alo, Moses, Ariom, Thaddeus, Ogbu, Ogbonnaya, Uneke Jesse, Chigozie   +3 more
core   +1 more source

Multifunctional Hydroxyapatite Coated with Gallium Liquid Metal‐Based Silver Nanoparticles for Infection Prevention and Bone Regeneration

Advanced Functional Materials, EarlyView.
A multifunctional hydroxyapatite (HAp) coating integrated with silver‐gallium liquid metal nanoparticles (HAp‐Ag‐GaNPs) exhibits dual antibacterial and osteogenic properties. It effectively inhibits Gram‐positive and Gram‐negative bacteria, including resistant strains, while enhancing bone regeneration.
Ngoc Huu Nguyen, Pengfei Zhang, Fathima Shana Pattar Kadavan, Zhaoning Xu, Tien Thanh Nguyen, Wenshao Li, Manh Tuong Nguyen, Chung Kim Nguyen, Duy Quang Pham, Thi Giang Tuyet Pham, Andrew Hayles, Markos Negash Alemie, Jitraporn Vongsvivut, Vincent Chan, Stephen Peter Kidd, Yunpeng Zhao, Krasimir Vasilev, Vi Khanh Truong   +17 more
wiley   +1 more source

Targeting B cells and plasma cells in autoimmune diseases: From established treatments to novel therapeutic approaches

European Journal of Immunology, Volume 53, Issue 1, January 2023., 2023
B lineage cells have been identified as therapeutic targets in autoimmune diseases. In the current review article, we provide an overview of novel strategies to target B lineage cells in autoimmune diseases, with a focus on inflammatory rheumatic diseases.
Ana Merino‐Vico, Giulia Frazzei, Jan Piet van Hamburg, Sander W. Tas   +3 more
wiley   +1 more source

Differences in antibiotic and antiviral use in people with confirmed influenza: a retrospective comparison of rapid influenza PCR and multiplex respiratory virus PCR tests

BMC Infectious Diseases, 2021
Background Influenza is a highly contagious respiratory virus with clinical impacts on patient morbidity, mortality and hospital bed management. The effect of rapid nucleic acid testing (RPCR) in comparison to standard multiplex PCR (MPCR) diagnosis in ...
Victor Au Yeung, Kiran Thapa, William Rawlinson, Andrew Georgiou, Jeffrey J. Post, Kristen Overton   +5 more
doaj   +1 more source