Results 41 to 50 of about 39,973 (307)

Evaluation of a non-animal toolbox informed by adverse outcome pathways for human inhalation safety

Frontiers in Toxicology
IntroductionThis work evaluated a non-animal toolbox to be used within a next-generation risk assessment (NGRA) framework to assess chemical-induced lung effects using human upper and lower respiratory tract models, namely MucilAir™-HF and EpiAlveolar ...
Renato Ivan de Ávila, Iris Müller, Hugh Barlow, Alistair Mark Middleton, Mathura Theiventhran, Danilo Basili, Anthony M. Bowden, Ouarda Saib, Patrik Engi, Tymoteusz Pietrenko, Joanne Wallace, Bernadett Boda, Samuel Constant, Holger Peter Behrsing, Vivek Patel, Maria Teresa Baltazar   +15 more
doaj   +1 more source

Alternatives to animal-derived extracellular matrix hydrogels? An explorative study with HepaRG cells in animal-free hydrogels under static and dynamic culture conditions

Frontiers in Toxicology
New approach methodologies (NAMs) aim for animal-free chemical risk assessments. However, many in vitro NAM models still depend heavily on Matrigel and collagen despite the ethical, reproducibility, and biomedical concerns regarding the use of animal ...
Katharina S. Nitsche, Paul L. Carmichael, Paul L. Carmichael, Sophie Malcomber, Iris Müller, Hans Bouwmeester   +5 more
doaj   +1 more source

Label-free highly sensitive probe detection with novel hierarchical SERS substrates fabricated by nanoindentation and chemical reaction methods

Beilstein Journal of Nanotechnology, 2019
Nanostructures have been widely employed in surface-enhanced Raman scattering (SERS) substrates. Recently, in order to obtain a higher enhancement factor at a lower detection limit, hierarchical structures, including nanostructures and nanoparticles ...
Jingran Zhang, Tianqi Jia, Yongda Yan, Li Wang, Peng Miao, Yimin Han, Xinming Zhang, Guangfeng Shi, Yanquan Geng, Zhankun Weng, Daniel Laipple, Zuobin Wang   +11 more
doaj   +1 more source

Comparison of Free-Space and Waveguide-Based SERS Platforms

Nanomaterials, 2019
Surface-Enhanced Raman Spectroscopy (SERS) allows for the highly specific detection of molecules by enhancing the inherently weak Raman signals near the surface of plasmonic nanostructures.
Nina Turk, Ali Raza, Pieter Wuytens, Hans Demol, Michiel Van Daele, Christophe Detavernier, Andre Skirtach, Kris Gevaert, Roel Baets   +8 more
doaj   +1 more source

Monolayer to MTS : using SEM, HIM, TEM and SERS to compare morphology, nanosensor uptake and redox potential in MCF7 cells

, 2015
This research received support from the QNano Project http://www.qnano-ri.eu which is financed by the European Community Research Infrastructures under the FP7 Capacities Programme (Grant No.
Campbell, C.J., C. J. Campbell, Bell, A.P., A. P. Bell, Jamieson, L.E., Harrison, D.J., L. E. Jamieson, D. J. Harrison   +7 more
core   +1 more source

Gold nanoparticles explore cells: cellular uptake and their use as intracellular probes [PDF]

, 2014
Understanding uptake of nanomaterials by cells and their use for intracellular sensing is important for studying their interaction and toxicology as well as for obtaining new biological insight.
Septiadi, Dedy, Barker, Roger A., Huefner, Anna, Fragniere, Alexandra, Mahajan, Sumeet, Kuan, Wei-Li, Wilts, Bodo D., Patel, Imran I.   +7 more
core   +1 more source

Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation

FEBS Letters, EarlyView.
Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe, Tanmay Kumar Mohanty, Rashna Bhandari, Dorothea Fiedler   +3 more
wiley   +1 more source

Sensitivity analysis of the inputs for bioactivity-exposure ratio calculations in a NAM-based systemic safety toolbox

NAM Journal
To support regulatory decision-making without animal testing, Next-Generation Risk Assessment (NGRA) frameworks leverage New Approach Methodologies (NAM). A widely used strategy in such frameworks is to calculate Bioactivity-Exposure Ratios (BERs), which
Hsing-Chieh Lin, Maria T Baltazar, Sophie Cable, Lucie C. Ford, Alistair M Middleton, Beate Nicol, Ans Punt, Joe Reynolds, Ivan Rusyn, Weihsueh A Chiu   +9 more
doaj   +1 more source

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

A Tunable Freeform-Segmented Reflector in a Microfluidic System for Conventional and Surface-Enhanced Raman Spectroscopy

Sensors, 2020
We present a freeform-segmented reflector-based microfluidic system for conventional Raman and Surface-Enhanced Raman Scattering (SERS) analysis. The segmented reflector is directly designed by a numerical approach.
Qing Liu, Michael Stenbæk Schmidt, Hugo Thienpont, Heidi Ottevaere   +3 more
doaj   +1 more source