Results 141 to 150 of about 709,020 (315)

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

Well-posedness in vector optimization and scalarization results [PDF]

In this paper, we give a survey on well-posedness notions of Tykhonov's type for vector optimization problems and the links between them with respect to the classification proposed by Miglierina, Molho and Rocca in [33].
Rocca Matteo, Papalia Melania
core  

Variational Inequalities Characterizing Weak Minimality in Set Optimization

, 2015
We introduce the notion of weak minimizer in set optimization. Necessary and sufficient conditions in terms of scalarized variational inequalities of Stampacchia and Minty type, respectively, are proved.
CRESPI, GIOVANNI PAOLO, Schrage, Carola, ROCCA, MATTEO   +2 more
core   +1 more source

A robust E learning recommendation system based on novel interval valued bipolar fuzzy hypersoft set theory

Scientific Reports
Understanding bipolar information is crucial as it enables individuals to make informed decisions that consider both extremes of a spectrum, leading to more balanced and effective outcomes.
Muhammad Imran Harl, Muhammad Saeed, Muhammad Haris Saeed, Muhammad Salman Habib, Mehran Ullah   +4 more
doaj   +1 more source

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Evolving dynamic multiple-objective optimization problems with objective replacement

, 2005
This paper studies the strategies for multi-objective optimization in a dynamic environment. In particular, we focus on problems with objective replacement, where some objectives may be replaced with new objectives during evolution.
Mo, W, Guan, SU, Chen, Q
core  

Vector optimization problems with linear criteria over a fuzzy combinatorial set of alternatives. [PDF]

, 2011
Vector optimization problems over a fuzzy combinatorial set of permutations are investigated. Based on the properties of the convex hull of a fuzzy combinatorial set of permutations, modifications of multicriteria choice methods are developed and ...
Koliechkina, L., Nagirna, A. M., Semenova, N. V., Semenova N. V., Nagirna A. M., Koliechkina L.   +5 more
core  

Optimization of Set-Valued Functions

Journal of Mathematical Analysis and Applications, 1994
zbMATH Open Web Interface contents unavailable due to conflicting licenses.
openaire   +2 more sources

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source