Results 231 to 240 of about 1,046,218 (306)

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Preventing Pesticide Toxicity Risk Through Self-Reported Practices in Children of Farming Communities: A Social Practice Theory Perspective. [PDF]

J Xenobiot
Nuraeni N, Herdiansyah H, Fatmah F, Agustina H, Yusuf R.   +4 more
europepmc   +1 more source

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Reevaluating Donor-recipient Sex Mismatch and Survival After Liver Transplantation: A Contemporary Cohort Study. [PDF]

Transplant Direct
Pollmann NS, Parmentier C, Rauchfuß F, Settmacher U, Pollmann L, Selzner M.   +5 more
europepmc   +1 more source

Several complex variables and complex geometry [proceedings of the Summer Research Institute on Several Complex Variables and Complex Geometry held at the University of California, Santa Cruz, Santa Cruz, California, July 10 - 30, 1989]

Bedford, Eric, Summer Research Institute on Several Complex Variables and Complex Geometry 1989 Santa Cruz, Calif.   +1 more
core  

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Graphene oxide nanoparticles oscillation in drilling fluid for heat transfer with entropy generation over radiative cylinder in fluctuating and laminar regimes. [PDF]

Discov Nano
Khedher NB, Ullah Z, Alamirew WD, Al Arni S, Elbadawi I, Khan I, Sarris IE, Amara MB, Boujelbene M.   +8 more
europepmc   +1 more source

C2α‐carbanion‐protonating glutamate discloses tradeoffs between substrate accommodation and reaction rate in actinobacterial 2‐hydroxyacyl‐CoA lyase

FEBS Open Bio, EarlyView.
Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn, Barbara Seroka, Ryszard Lazny, Zenon Lotowski, Thore Rohwerder   +4 more
wiley   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Large language models for predicting postoperative complications: benefits, potential, and limitations of clinical use. [PDF]

Korean J Anesthesiol
Kwon YS.
europepmc   +1 more source