Cleavable N‐terminal Thioredoxin fusion enabled soluble expression and purification of otherwise insoluble SARS‐CoV‐2 Nucleocapsid (N) protein. A four‐step purification strategy yielded highly homogeneous, RNA‐free N protein. Binding assays showed high RNA affinity (Kd ~ 28 nm). The study will facilitate high‐resolution structural studies of N protein,
Shweta Singh, Gagan D. Gupta
wiley +1 more source
Coronavirus Disease 2019 Lineages in a Minimally Vaccinated Island Population: Genomic Epidemiology of Severe Acute Respiratory Syndrome Coronavirus 2 in Haiti. [PDF]
Mavian CN +15 more
europepmc +1 more source
Implementation of FTA Elute Card for Viral RNA Storage and Cost‐Effective Wastewater Surveillance
A strategy is developed for room‐temperature storage of RNA from wastewater using FTA Elute cards, which allow for easy and portable transportation. FTA Elude cards provided a 15.4% recovery of the original RNA with cards, compared to only 0.5% recovery without cards.
Qingxin Hui +3 more
wiley +1 more source
Effect of Universal Masking on Non-Severe Acute Respiratory Syndrome Coronavirus 2 Healthcare-Associated Respiratory Viral Infections. [PDF]
Munigala S +8 more
europepmc +1 more source
The NSP5, ORF6 and NSP13 of SARS‐CoV‐2 Cooperate to Modulate Inflammatory Cell Death Activation
ZBP1 is capable of initiating a large cell death complex to induce programmed cell death during SARS‐CoV‐2 infection. However, SARS‐CoV‐2 can inhibit the activation of ZBP1‐mediated cell death by targeting key components of this complex. This suppression of ZBP1‐mediated cell death may account for the increased mortality observed in patients co ...
Huan Wang +16 more
wiley +1 more source
Trajectories of Health-Related Quality of Life 2 Years After Mild/Moderate Severe Acute Respiratory Syndrome Coronavirus 2 Infection in the Pre-Omicron Era. [PDF]
Jacka BP +6 more
europepmc +1 more source
Interim Report of the Reactogenicity and Immunogenicity of Severe Acute Respiratory Syndrome Coronavirus 2 XBB-Containing Vaccines. [PDF]
Chalkias S +17 more
europepmc +1 more source

