Results 201 to 210 of about 36,869 (295)

Functional validation of a white pupae minimal gene construct in Ceratitis capitata (Diptera: Tephritidae)

Insect Science, EarlyView.
An intronless version of the white pupae (wp) gene was engineered to restore the wild type brown puparium color in white pupae phenotype mutants of the Mediterranean fruit fly, Ceratitis capitata. Functionality of the minimal gene version (mini‐wp) was verified in vivo, as one copy of mini‐wp successfully restored the wild type phenotype in homozygous ...
Lucas Henrique Figueiredo Prates, Roswitha A. Aumann, Inga Sievers, Tanja Rehling, Marc F. Schetelig   +4 more
wiley   +1 more source

Genetic evaluation of CRISPR-Cas9 off-target effects from deleterious mutations on Drosophila male single X chromosome. [PDF]

Funct Integr Genomics
Bian W, Mcquarrie DWJ, Haussmann IU, Arnold R, Soller M.   +4 more
europepmc   +1 more source

Molecular mechanisms of sex determination in Lepidoptera: current status and perspectives

Insect Science, EarlyView.
The genetic basis of sex determination in Lepidoptera was discovered in 2014 in the silkworm Bombyx mori. In this model species, the W chromosome‐derived small piRNA called Fem piRNA downregulates the expression of a Z‐linked gene, Masculinizer (Masc), which leads to the default female‐specific splicing of the doublesex gene (dsxF) and thus to female ...
František Marec, Atsuo Yoshido, Arjen E. van′t Hof   +2 more
wiley   +1 more source

Cytogenetic landscape aberrations in paediatric acute lymphoblastic leukaemia - a polish paediatric population treated according to ALL-IC BFM 2009 protocol. [PDF]

Sci Rep
Lejman M, Styka B, Zawitkowska J, Pastwińska A, Studniak E, Skonieczka K, Zacharczuk M, Całka K, Haus O, Barbara P, Sadowska B, Przybyłowicz-Chalecka A, Jarmuż-Szymczak M, Malm M, Drop B, Kowalczyk JR.   +15 more
europepmc   +1 more source

The shibirets4 mutation causes temperature sensitive paralytic and lethal phenotypes in the Queensland fruit fly, Bactrocera tryoni

Insect Science, EarlyView.
Abstract Bactrocera tryoni, the Queensland fruit fly, is among the most damaging insect pests to the Australian horticultural industry as larvae infest ripening fruits or vegetables prior to harvest. Genetic biocontrol using Sterile Insect Technique (SIT) programs have been used to successfully suppress populations, via mass release of factory‐reared ...
Anzu Okada, Mamoru Okamoto, Thu N.M. Nguyen, Elisabeth Fung, Han Nguyen, Peter Crisp, Amanda Choo, Simon W. Baxter   +7 more
wiley   +1 more source

Clinical and economic impact of genome-wide non-invasive prenatal testing (NIPT) as a first-tier screening method compared to targeted NIPT and first-trimester combined testing: A modeling study. [PDF]

PLoS Med
van Prooyen Schuurman L, de Koning HJ, Meier E, Galjaard RH, van Ravesteyn NT.   +4 more
europepmc   +1 more source

Development and evaluation of pupal color‐based genetic sexing strains in Anastrepha obliqua (Diptera: Tephritidae)

Insect Science, EarlyView.
Two spontaneous mutations in pupae of the West Indian fruit fly Anastrepha obliqua, named bp and sp, were isolated and studied. The bp mutation was selected, and using two types of Y‐autosoma l translocation inducers and an appropriate crossing scheme, two genetically sexed strains based on pupa l color were developed for the first time in this species,
Daisy P. Cárdenas‐Enríquez, Víctor García‐Martínez, Jorge Ibáñez‐Palacios, Brenda Torres‐Huerta, Maria F. Ruiz‐Pérez, José S. Meza   +5 more
wiley   +1 more source

Epimutation analysis reveals involvement of SLIT2/ROBO signaling pathway in painful diabetic neuropathy. [PDF]

Hum Genomics
Kwiatkowska KM, Garagnani P, Ferraresi F, Bonafé M, Bacalini MG, Sala C, Castellani G, Gentilini D, Calzari L, Ziegler D, Gerrits MM, Faber CG, Malik RA, Marchi M, Salvi E, Lauria G, Pirazzini C.   +16 more
europepmc   +1 more source

DsiRNA‐mediated silencing of Ceratitis capitata transformer or transformer‐2 leads to masculinization of XX embryos and systemic gene silencing in ovaries

Insect Science, EarlyView.
We carried out functional studies using Dicer‐substrate small interfering RNAs (DsiRNAs), targeting the sex determination genes Cctransformer (Cctra) and Cctransformer‐2 (Cctra‐2) in the Mediterranean fruit fly (Ceratitis capitata). Microinjection of DsiRNAs into XX embryos (0‐1 h old) rapidly triggered the expression of male‐specific Cctra isoforms ...
Gennaro Volpe, Sarah Maria Mazzucchiello, Domenico De Falco, David Torrente, Stefania Liguori, Noemi Rosati, Dora Baccaro, Michela Mazzeo, Fulvio Bertolotto, Harshini Sangle, Ennio Giordano, Angela Carfora, Francesca Lucibelli, Marianna Varone, Paola Di Lillo, Hugo Diego Perdomo, Mariangela Bonizzoni, Serena Aceto, Marco Salvemini, Molly Duman‐Scheel, Giuseppe Saccone   +20 more
wiley   +1 more source

Spermatocyte injection into meiotic oocytes rescues diplotene, but not pachytene, arrest in azoospermic mutant mice. [PDF]

Hum Reprod Open
Ogonuki N, Hino T, Fujiwara Y, Osawa Y, Mizuno S, Sugiyama F, Kunieda T, Otsuki J, Oura S, Furuse T, Okada Y, Tamura M, de la Casa-Esperon E, Ikawa M, Inoue K, Ogura A.   +15 more
europepmc   +1 more source