Results 1 to 10 of about 21,909 (100)
Lysis of Mammalian and Sf9 Cells [PDF]
, 2014 Use a French press to disrupt mammalian or Sf9 cells and generate a clarified lysate for subsequent use in protein purification.
Jennifer M, Kavran, Daniel J, Leahy
openaire +2 more sources
Ethanol Stimulates Expression of Functional H+,K+-ATPase in SF9 Cells [PDF]
Biochemical and Biophysical Research Communications, 1995 The baculovirus expression system is suitable for functional expression of gastric H+,K(+)-ATPase. Expression of functional H+,K(+)-ATPase in Sf9 cells is accompanied by synthesis of large amounts of non-functional subunits. When H+,K(+)-ATPase is synthesised in the presence of 150-250 mM ethanol in the culture medium, two to threefold higher levels of
Klaassen, C.H.W. +2 more
openaire +4 more sources
Multiple factors conferring high radioresistance in insect Sf9 cells [PDF]
Mutagenesis, 2009 Sf9, a lepidopteran cell line isolated from the fall armyworm, Spodoptera frugiperda, was shown to be significantly more resistant to growth inhibition and apoptosis induction effects of x-ray irradiation than several human cell lines of different origins.
I-Cheng, Cheng, How-Jing, Lee, T C, Wang
openaire +2 more sources
Binding specificity of ostreolysin A6 towards Sf9 insect cell lipids
Biochimica et Biophysica Acta (BBA) - Biomembranes, 2020 Oyster mushrooms (Pleurotus spp.) have recently been shown to produce insecticidal bi-component protein complexes based on the aegerolysin proteins. A role for these proteins is thus indicated for defence and protection of the mushroom, and we propose their use as new environmentally friendly bioinsecticides.
Novak M. +7 more
openaire +4 more sources
Nanostructural characterization of Sf9 cells during virus‐like particles generation [PDF]
Scanning, 2016 SummarySf9 cells (a clonal isolate of Spodoptera frugiperda Sf21 cells) are commonly used to generate recombinant virus‐like particles (VLPs). For VLPs generation, Sf9 cells are infected with recombinant baculoviruses (rBV) expressing desired proteins. During rBV infections, Sf9 cells have changes in cell diameters and surface structures. In this study,
Gi-Ja, Lee +3 more
openaire +2 more sources
Immunogenicity of HGV NS5 protein expressed from Sf9 insect cells
World Journal of Gastroenterology, 2001 Although reliable assays for the detection of hepatitis C virus and E virus became available, still 10%-20% hepatitis are not caused by hepatitisA-E virus[1-3]. In 1996, two research groups isolated this agent independently and almost simultaneously and named hepatitis G virus and GB virus C, respectively[4-7].
H, Ren +4 more
openaire +2 more sources
Receptor-mediated activation of recombinant Trpl expressed in Sf9 insect cells [PDF]
Biochemical Journal, 1995 The Drosophila proteins, Trp and Trpl, are suggested to be cation channels responsible for depolarization of the receptor potential associated with stimulation of insect photoreceptor cells by light. Consistent with this hypothesis, we recently showed that recombinant Trpl forms Ca(2+)- and Ba(2+)-permeable non-selective cation channels when expressed ...
Y, Hu, W P, Schilling
openaire +2 more sources
Study of the improved Sf9 transient gene expression process [PDF]
BMC Proceedings, 2013 Insect cells have been widely used for the production of recombinant proteins using recombinant baculovirus for gene delivery [1]. To simplify protein production in insect cells, we have previously described a method, based on transient gene expression (TGE) with cultures of suspension-adapted Sf9 cells using polyethylenimine (PEI) for DNA delivery [2].
Shen, Xiao +3 more
openaire +1 more source
Expression and characterization of two STAT isoforms from Sf9 cells
Developmental & Comparative Immunology, 2008 In invertebrates, the JAK-STAT signaling pathway is involved in the anti-bacterial response and is part of an anti-viral response in Drosophila. In this study, we show that two STAT transcripts are generated by alternative splicing and encode two isoforms of Sf-STAT with different C-terminal ends. These two isoforms were produced and purified using the
Maw-Sheng, Yeh +8 more
openaire +2 more sources
Cell-Cycle Perturbation in Sf9 Cells Infected withAutographa californicaNucleopolyhedrovirus
Virology, 1999 Flow cytometry analysis of the cell-cycle progression was performed in Sf9 cells infected with Autographa californica nucleopolyhedrovirus (AcNPV) in the cultures partially synchronized by aphidicolin exposure and deprivation. Cells infected with AcNPV during the G1 phase progressed and were arrested in the S phase in the 4 h following the infection ...
Ikeda, Motoko, Kobayashi, Michihiro
openaire +2 more sources