Results 141 to 150 of about 22,318 (164)
Some of the next articles are maybe not open access.
Functional Expression of His-Tagged Rhodopsin in Sf9 Insect Cells
2003Item does not contain ...
Bosman, G.J.C.G.M. +5 more
openaire +4 more sources
Radioresistant Sf9 insect cells display moderate resistance against cumene hydroperoxide
Molecular and Cellular Biochemistry, 2012Lepidopteran insect cells serve as excellent model to study stress responses and are known to display resistance against DNA damaging agents including ionizing radiation; however, limited information is available on the effects of membrane damaging agents in these cells.
Jyoti Swaroop, Kumar +3 more
openaire +2 more sources
The Wilson's disease protein expressed in Sf9 cells is phosphorylated
Biochemical Society Transactions, 2002The Wilson's disease protein (WNDP), a copper transporter, is a crucial mediator of copper homoeostasis in mammalian cells. We recently found that changes in copper concentration regulate the phosphorylation level of WNDP. WNDP phosphorylation was observed in several mammalian cell lines, suggesting that a common phosphorylation pathway exists in these
S M, Vanderwerf, S, Lutsenko
openaire +2 more sources
Purification of Functional Recombinant YY1 Expressed in Sf9 Insect Cells
Protein Expression and Purification, 1995We expressed a gene for YY1, the multifunctional mammalian transcription factor, with a recombinant baculovirus in insect cells and obtained high levels of protein in cell lysates. A simple and efficient purification method was developed. In the final step, we used DNA affinity chromatography with concatermerized sequence derived from the upstream ...
M, Momoeda +3 more
openaire +2 more sources
Activation of recombinant trp by thapsigargin in Sf9 insect cells
American Journal of Physiology-Cell Physiology, 1994The mammalian protein responsible for Ca2+ release-activated current (Icrac) may be homologous to the Drosophila protein designated trp. Thus the activity of trp, and another Drosophila protein designated trp-like or trpl, may be linked to depletion of the internal Ca2+ store via the so-called capacitative Ca2+ entry mechanism. To test this hypothesis,
L, Vaca +4 more
openaire +2 more sources
Single-Molecule Analysis of Sf9 Purified Superprocessive Kinesin-3 Family Motors
Journal of Visualized Experiments, 2022A complex cellular environment poses challenges for single-molecule motility analysis. However, advancement in imaging techniques have improved single-molecule studies and has gained immense popularity in detecting and understanding the dynamic behavior of fluorescent-tagged molecules.
Pushpanjali, Soppina +3 more
openaire +2 more sources
Endogenous K+/H+ Exchange Activity in the Sf9 Insect Cell Line
Biochemistry, 1995The fluorescent pH indicator 2',7'-bis(carboxyethyl)-5,6-carboxyfluorescein (BCECF) was used to investigate changes in the intracellular pH (pHi) of individual Sf9 cells in response to changes in the composition of the external medium. Under standard conditions, the resting pHi was 0.2-0.3 unit lower than the extracellular pH (6.5).
Vachon, Vincent +4 more
openaire +3 more sources
φC31 ‐Mediated cassette exchange in Sf9 insect cells for stable expression
Biotechnology Journal, 2023AbstractInsect cells, especially Sf9 cells, are commonly used in biomanufacturing due to their advantages in high expression levels and post‐translational modification. However, the development of stable expression cell lines via random integration tended to be unstable. Site‐specific integration (SSI) is an alternative strategy. In this study, a φC31 ‐
Die Hu +6 more
openaire +2 more sources
Gbetagamma-copurified lipid kinase impurity from Sf9 cells.
Protein and peptide letters, 2009G-protein betagamma dimers are prime regulators transmitting extracellular signals to wide-ranging cellular effectors including phosphoinositide 3-kinase (PI3K) isoforms beta and gamma. Recombinant Gbetagamma purified from Sf9 cells via metal-affinity and anion exchange chromatography exhibited a wortmannin-insensitive phospholipid kinase activity that
Aliaksei, Shymanets +2 more
openaire +2 more sources
Functional expression of solubleicam-1 by baculovirus-infected Sf9 cells
Biochemical and Biophysical Research Communications, 1992Inflammation, metastasis and ischemia are processes that require lymphocyte or leukocyte cell recognition and adherence to endothelial counter receptors such as ICAM-1. Mapping the sites of interaction of ICAM-1 with LFA-1, the receptor for ICAM-1 on lymphocytes, may lead to the design of novel inhibitors of inflammation or metastasis.
R R, Cobb +3 more
openaire +2 more sources