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Expression and Purification of Dengue NS1 Protein in Sf9-Baculovirus System
2021It is well known that glycosylations of Dengue NS1 protein are important for its structure, oligomerization, and immunogenicity. One of the major challenges in heterologous NS1 protein expression is the difference in glycosylation patterns amongst different organisms.
Diego R, Coelho +1 more
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Assays of Recombinant Adenylyl Cyclases Expressed in Sf9 Cells
2003This chapter outlines procedures for the expression of mammalian membrane-bound adenylyl cyclases (AC) in Sf9 cells and subsequent in vitro methods for assessing the activity of these cyclases. Membrane preparations derived from this overexpression system provide homogeneous sources of mammalian AC because AC that are endogenously expressed in Sf9 ...
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Eextracellular Histone H4 from SF9 Cells Is Antimicrobial
2005Spodoptera frugiperda Sf9 cells produces an extracellular, truncated form of histone H4 (H4-N12). H4-N12 was suspected to have antimicrobial effects. To investigate this, conditioned medium from Sf9 cell cultures was concentrated and fractioned on a gel filtration column. The fractions containing H4-N12, were collected, pooled and the buffer exchanged (
K. Calles +4 more
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Radiation responses of Sf9, a highly radioresistant lepidopteran insect cell line
International Journal of Radiation Biology, 2004Lepidopteran insect cells are known to exhibit very high radioresistance. Although very effective DNA excision-repair has been proposed as a contributing factor, a detailed understanding of insect cell radiation responses has not yet been obtained. Therefore, the study was carried out to understand the in vitro radiation responses of Sf9 lepidopteran ...
S, Chandna +5 more
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Homogeneous Purification of Human Recombinant GST–Akt/PKB from Sf9 Cells
Protein Expression and Purification, 1999The plethora of extracellular stimuli modulating the status of a cell results in the engagement of relatively few pathways responsible for transducing signals to the interior of the cell. One such pathway is the activation of phosphatidylinositol 3-kinase (PI-3K), which results in the generation of a membrane-restricted second messenger ...
D, Fabbro +5 more
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Characterization of human 5-HT1 receptors expressed in Sf9 insect cells
European Journal of Pharmacology: Molecular Pharmacology, 1994Four human 5-HT receptor subtypes (5-HT1A, 5-HT1D alpha, 5-HT1D beta and 5-HT1E) have been expressed in Sf9 insect cells. All four human 5-hydroxytryptamine receptors produced by Sf9 cells had the expected pharmacological properties. Surprisingly, levels of expression of these receptors were relatively low (1-5 pmol/mg protein).
E M, Parker +6 more
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Silkworm hemolymph as a potent inhibitor of apoptosis in Sf9 cells
Biochemical and Biophysical Research Communications, 2002We have previously shown that silkworm hemolymph exhibits anti-apoptotic activity against baculovirus-induced Sf9 cell apoptosis. In this study, using various chemical inducers, such as actinomycin D, camptothecin, and staurosporine, we found that silkworm hemolymph inhibits insect cell apoptosis induced not only by baculovirus but also by chemical ...
Won Jong, Rhee +2 more
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Solubilization of muscarinic receptor subtypes from baculovirus infected sf9 insect cells
Biochemical Pharmacology, 1994Five different subtypes (human m1, m2, m5 and rat m3, m4) of muscarinic acetylcholine receptors (mAChR) were produced in insect Sf9 cells by infection with recombinant baculoviruses. N-[3H]methylscopolamine ([3H]NMS) has a similar affinity to each of these mAChR subtypes in cell membranes, while pirenzepine, 11-((2-[(diethylamino)methyl]-1-piperidinyl ...
A, Rinken +3 more
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[Localization of AcMNPV NLA genes in Sf9 cells].
Bing du xue bao = Chinese journal of virology, 2012Nuclear actin which plays a key role in many nucleic processes has become a research hotspot. Baculovirus is the only reported pathogen using nuclear actin to replicate and proliferate. However, little is known about the mechanism of monomeric G-actin accumulation within nuclei of baculovirus-infected cells. It has been reported that AcMNPV ie-1, pe38,
Qian, Wang +4 more
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Purification and Characterization of Aspartic Protease Derived from Sf9 Insect Cells
Bioscience, Biotechnology, and Biochemistry, 2010An aspartic protease that is significantly produced by baculovirus-infected Spodoptera frugiperda Sf9 insect cells was purified to homogeneity from a growth medium. To monitor aspartic protease activity, an internally quenched fluoresce (IQF) substrate specific to cathepsin D was used.
Takeshi, Gotoh +4 more
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