Results 11 to 20 of about 22,318 (164)
Addictive Behaviors Reports, 2021 Background: Associations between internet gaming disorder (IGD), psychological distress, and sleep have been reported. However, little is known whether such associations exist across siblings; that is, whether adolescents’ IGD symptomatology may impact ...
Chung-Ying Lin +3 more
doaj +1 more source
Plant Virus-Induced Inheritable Apoptosis Drives Reproductive Costs in Female Insect Vectors to Balance Viral Biparental Transmission. [PDF]
Adv Sci (Weinh)Rice gall dwarf virus (RGDV), transmitted biparentally by leafhopper vector to their offspring, exploits the capsid protein to induce inheritable ovarian apoptosis via insulin‐driven PI3K/AKT/FoxO signaling axis. This mechanism enhances infection but reduces female reproductive fitness, causing inefficient maternal transmission.
Wu H +6 more
europepmc +2 more sources
Protease-mediated maturation of M-PMV reverse transcriptase into a functional heterodimer. [PDF]
Protein SciAbstract Reverse transcriptase (RT) of retroviruses orchestrates viral replication, yet its structural diversity remains poorly understood. Well‐studied RTs, such as those from HIV‐1, murine leukemia virus, and avian myeloblastosis virus, were characterized decades ago, but less prominent retroviruses have escaped detailed analysis.
Kapisheva M +6 more
europepmc +2 more sources
Chimeric virus-like particles carrying the CLEC17A carbohydrate-recognition domain significantly reduce Macrobrachium rosenbergii nodavirus infection in Sf9 cells. [PDF]
Sci RepOur previous studies demonstrated that Macrobrachium rosenbergii nodavirus (MrNV) infects and replicates in Sf9 insect cells, where fucosylated N- and O-glycans (HexNAc (Fuc)HexNAc-R or Fuc-LacdiNAc) serve as essential binding molecules for viral ...
Chantunmapitak R +7 more
europepmc +2 more sources
Rapid and Economic Baculovirus Titer Determination Using a Novel Transgenic Sf9-QE Cell Line. [PDF]
InsectsA baculovirus expression system (BES) for the production of recombinant proteins requires rapid and easy virus titer determination. In this study, a novel direct titration method was developed using a novel Sf9-QE cell line to easily and economically ...
Moon HJ, Kim HJ, Lee DH, Mun SY, Woo SD.
europepmc +2 more sources
Expression and Purification of H7 Subtype Avain Influenza Viruses HA Protein in Sf9
Guangdong nongye kexue, 2023 【Objective】The experiment is aimed to express the HA protein of H7 subtype avian influenza virus (AIV) in eukaryotic cells, in order to provide bass for futher preparation of H7 subunit vaccine and evaluation of their immunogenicity.【Method】First, the ...
Yufu LIU +4 more
doaj +1 more source
Tracking the virus-like particles of Macrobrachium rosenbergii nodavirus in insect cells [PDF]
PeerJ, 2017 Macrobrachium rosenbergii nodavirus (MrNv) poses a major threat to the prawn industry. Currently, no effective vaccine and treatment are available to prevent the spread of MrNv.
Ummi Fairuz Hanapi +5 more
doaj +2 more sources
Virulence, 2022 The vegetative insecticidal proteins (Vip3A) secreted by some Bacillus thuringiensis (Bt) strains during vegetative growth are regarded as a new generation of insecticidal toxins. Like insecticidal crystal proteins, they are also used in transgenic crops
Baoju An +5 more
doaj +1 more source
An improved CRISPR-Cas9 protein-based method for knocking out insect Sf9 cell genes. [PDF]
Appl Microbiol BiotechnolInsect cells are one of the uprising expression systems in the biopharmaceutical industry to produce vaccines and gene therapy vectors, but cell line development has been limited by the lack of established genetic engineering tools and genomic ...
Graça M +4 more
europepmc +2 more sources
Frontiers in Public Health, 2023 BackgroundIn its most recent edition of the Diagnostic and Statistical Manual of Mental Disorders (DSM), the American Psychological Association recognized that internet gaming disorder is a psychological condition and provides nine criteria for its ...
Mogeda El Sayed El Keshky +1 more
doaj +1 more source