The mitochondria-mediate apoptosis of Lepidopteran cells induced by azadirachtin. [PDF]
PLoS ONE, 2013 Mitochondria have been shown to play an important role in apoptosis using mammalian cell lines. However, this seems not to be the case in Drosophila, an insect model organism; thus more in-depth studies of insect cell apoptosis are necessary.
Jingfei Huang +3 more
doaj +1 more source
DSP-PP precursor protein cleavage by tolloid-related-1 protein and by bone morphogenetic protein-1.
PLoS ONE, 2012 Dentin sialoprotein (DSP) and phosphophoryn (PP), acidic proteins critical to dentin mineralization, are translated from a single transcript as a DSP-PP precursor that undergoes specific proteolytic processing to generate DSP and PP.
Helena H Ritchie +4 more
doaj +1 more source
Oxidative stress and DNA damage induced by spinosad exposure in Spodoptera frugiperda Sf9 cells
Food and Agricultural Immunology, 2018 Spinosad, a neurotoxic insecticide, is widely used for crop protection. In order to elucidate the effects of spinosad on oxidative stress and genotoxicity in Sf9 cells, the levels of lipid peroxidation, the activity of antioxidative enzymes, and DNA ...
Wenping Xu +4 more
doaj +1 more source
Improved Expression of SARS-CoV-2 Spike RBD Using the Insect Cell-Baculovirus System
Viruses, 2022 Insect cell-baculovirus expression vector system is one of the most established platforms to produce biological products, and it plays a fundamental role in the context of COVID-19 emergency, providing recombinant proteins for treatment, diagnosis, and ...
Joaquín Poodts +10 more
doaj +1 more source
Production and characterization of novel recombinant adeno-associated virus replicative-form genomes: a eukaryotic source of DNA for gene transfer. [PDF]
PLoS ONE, 2013 Conventional non-viral gene transfer uses bacterial plasmid DNA containing antibiotic resistance genes, cis-acting bacterial sequence elements, and prokaryotic methylation patterns that may adversely affect transgene expression and vector stability in ...
Lina Li +9 more
doaj +1 more source
Performance of Cardiotropic rAAV Vectors Is Dependent on Production Method
Viruses, 2022 Gene therapy is making significant impact on a modest, yet growing, number of human diseases. Justifiably, the preferred viral vector for clinical use is that based on recombinant adeno-associated virus (rAAV).
Renuka Rao +7 more
doaj +1 more source
A Novel Transgenic Sf9 Cell Line for Quick and Easy Virus Quantification
InsectsThe following study was conducted to generate a transgenic Sf9 cell line for rapid and easy virus quantification in the baculovirus expression system (BES). The hr3 (homologous region 3) and 39K and p10 promoters were used as the expression structures to
Kyu-Seek Kim +4 more
doaj +1 more source
PLoS ONE, 2018 The human Adeno-Associated Virus serotype 2 (wtAAV2) is a common non-pathological virus and its recombinant form (rAAV) is widely used as gene therapy vector. Although rAAVs are routinely produced in the Baculovirus/Sf9 cell system, wtAAV2 has never been
Adrien Savy +5 more
doaj +1 more source
STAR Protocols, 2023 Summary: The interaction manner and biological function of Rab7 and its effector, Rab-interacting lysosomal protein (RILP), remain unclear in invertebrates.
Gaofeng Cui, Zhiyan Jiang, Guohua Zhong
doaj +1 more source
Kinetic analysis of in vitro production of wild-type Spodoptera frugiperda nucleopolyhedrovirus
Brazilian Archives of Biology and Technology, 2010 In this study, the kinetic behavior of Sf9 and Sf21 cells used in the production of a baculovirus biopesticide to control the pest of corn Spodoptera frugiperda was analyzed. Kinetic variables such as maximum specific growth rate, cell productivity, mean
Andréa Farias de Almeida +3 more
doaj +1 more source