Results 71 to 80 of about 22,318 (164)

Allosteric Inhibitors of SARS‐CoV‐2 RNA‐Dependent RNA Polymerase Based on N,N′‐Diphenylurea

ChemMedChem, Volume 21, Issue 1, January 2026.
Inhibitors of SARS‐CoV‐2 RdRp activity based on N,N′‐diphenylurea scaffold have been identified. Several compounds exhibit residual activity (RA) below 50% in an enzymatic assay (at 10 µM concentration) and improved aqueous solubility compared to the previously reported series of inhibitors.
Artem Chayka, Matěj Danda, Alžběta Dostálková, Vojtěch Spiwok, Lamija Zijadic, Anna Klimešová, Marina Kapisheva, Michala Zgarbová, Jan Weber, Tomáš Ruml, Michaela Rumlová, Zlatko Janeba   +11 more
wiley   +1 more source

Production and crystallization of lobster muscle tropomyosin expressed in Sf9 cells

FEBS Letters, 1996
A new form of muscle tropomyosin crystal has been obtained, by employing new strategies in protein preparation and crystallization. Non‐polymerizable tropomyosin was prepared by removing 11 amino acids at the C‐terminus. The truncated tropomyosin was expressed in Sf9 insect cells by use of the baculovirus‐based expression system, to obtain highly ...
Miegel, Andrea, Sano, Ken-Ichi, Yamamoto, Kazuhiro, Maeda, Kayo, Maéda, Yuichiro, Taniguchi, Hisaaki, Yao, Min, Wakatsuki, Soichi   +7 more
openaire   +2 more sources

Cloning, Expression, and Purification of the Recombinant Hemagglutinin of Human Influenza Virus H1N1 in the Eukaryotic Insect Cells Using Baculovirus Vector

مجله دانشکده پزشکی اصفهان, 2020
Background: The H1N1 influenza virus is a highly pathogenic virus that threatens human life. Vaccination is an effective way of preventing and controlling influenza.
Niloufar Rashedi, Morteza Taghizadeh, Parisa Mohamadynejad, Mehdi Mahdavi, Reza Jalalirad   +4 more
doaj   +1 more source

Purification and characterization of recombinant human translation initiation factor eIF3

Protein Science, Volume 35, Issue 1, January 2026.
Abstract Eukaryotic translation initiation factor 3 (eIF3) is an essential factor in protein synthesis. In mammals, it is a ~800 kDa complex composed of 12–13 subunits. Biochemical and mechanistic studies of the function of eIF3 and its individual subunits in translation require purified eIF3.
Irene Diaz‐Lopez, Yuliya Gordiyenko, Philipp K. Zuber, Xueyan Li, V. Ramakrishnan   +4 more
wiley   +1 more source

Human Papillomavirus Type16- L1 VLP Production in Insect Cells [PDF]

Iranian Journal of Basic Medical Sciences, 2013
Objective(s):  Infection by high-risk papillomavirus is regarded as the major risk factor in the development of cervical cancer. Recombinant DNA technology allows expression of the L1 major capsid protein of HPV in different expression systems, which has
Asghar Abdoli, Hoorieh Soleimanjahi, Fatemeh Fotouhi, Shahram Pour Beiranvand, Zahra Kianmehr   +4 more
doaj  

Activity‐restoring mutations in the histamine H3 receptor increase constitutive activity and reduce structural stability

Protein Science, Volume 35, Issue 1, January 2026.
Abstract The histamine H3 receptor (H3R) is a class A G protein‐coupled receptor (GPCR) that regulates neurotransmitter release in the central nervous system. Although structures of H3R in both inactive and active states have been elucidated, the functional roles of specific residues remain unclear.
Ami Nakajima, Hiroto Kaneko, Kosuke Oyama, Misumi Kuchiji, Ayane Itakura, Chiaki Arai, Mitsunori Shiroishi   +6 more
wiley   +1 more source

Defining substrate specificities of human RNA capping methyltransferases through quantitative assessment of independent yet cooperative activities

Protein Science, Volume 35, Issue 1, January 2026.
Abstract Human RNA capping is critical for mRNA splicing, protection of RNA from 5′ exonucleases in the cytoplasm, and targeting to the ribosome. Human RNMT, CMTR1, and CMTR2 are RNA methyltransferases involved in the RNA capping process. They play a significant role in the proliferation and differentiation of embryonic stem cells and have been ...
Fatemeh Taherian, Mark F. Mabanglo, Taraneh Hajian, Sarah Tucker, Emilija Kalinic, Sumera Perveen, Ahmed Aman, Masoud Vedadi   +7 more
wiley   +1 more source

Preparation of ATP13A2 microsomes from Sf9 cells v1

, 2022
Isolate microsomes from Sf9 cells expressing ATP13A2
openaire   +1 more source

Intracellular Trafficking of Baculovirus Particles: A Quantitative Study of the HearNPV/HzAM1 Cell and AcMNPV/Sf9 Cell Systems

Viruses, 2015
To replace the in vivo production of baculovirus-based biopesticides with a more convenient in vitro produced product, the limitations imposed by in vitro production have to be solved.
Leila Matindoost, Lars K. Nielsen, Steve Reid   +2 more
doaj   +1 more source

Ao38, a new cell line from eggs of the black witch moth, Ascalapha odorata (Lepidoptera: Noctuidae), is permissive for AcMNPV infection and produces high levels of recombinant proteins

BMC Biotechnology, 2010
Background The insect cell line is a critical component in the production of recombinant proteins in the baculovirus expression system and new cell lines hold the promise of increasing both quantity and quality of protein production. Results Seventy cell
Zhang Sheng, Hashimoto Yoshifumi, Blissard Gary W   +2 more
doaj   +1 more source