Pieris rapae is one of the most destructive pests to cabbage around the world and it has developed resistance to present pesticides. 5′-Epi-SPA-6952A was a new 24-membered macrolide isolated from the cultured broth of Streptomyces diastatochromogenes ...
Yang Zhang +7 more
doaj +1 more source
Recombinant Proteins: A Molecular Tool to Understand Marine Adhesion and to Advance Biomaterials
The production of recombinant proteins represents a fundamental step in the characterisation of marine invertebrate adhesives and in the development of bio‐inspired glues. The association of these proteins with other components such as ions, proteins, polysaccharides, or polymers enables the fabrication of biomaterials for various healthcare ...
Alessandra Whaite +4 more
wiley +1 more source
The Nuclease Activity of the Yeast Dna2 Protein, Which Is Related to the RecB-like Nucleases, Is Essential in Vivo [PDF]
Saccharomyces cerevisiae Dna2 protein is required for DNA replication and repair and is associated with multiple biochemical activities: DNA-dependent ATPase, DNA helicase, and DNA nuclease.
Budd, Martin E. +2 more
core +1 more source
Whole-Genome Sequence of the Spodoptera frugiperda Sf9 Insect Cell Line [PDF]
ABSTRACT The draft whole-genome sequence of the Spodoptera frugiperda Sf9 insect cell line was obtained using long-read PacBio sequence technology and Canu assembly. The final assembled genome consisted of 451 Mbp in 4,577 contigs, with 12,716× mean coverage and a G+C content of 36.53%.
Nandakumar, Subhiksha +2 more
openaire +2 more sources
Specific recognition of a multiply phosphorylated motif in the DNA repair scaffold XRCC1 by the FHA domain of human PNK. [PDF]
Short-patch repair of DNA single-strand breaks and gaps (SSB) is coordinated by XRCC1, a scaffold protein that recruits the DNA polymerase and DNA ligase required for filling and sealing the damaged strand.
Ali, Ammar A. E. +3 more
core +3 more sources
Allosteric Inhibitors of SARS‐CoV‐2 RNA‐Dependent RNA Polymerase Based on N,N′‐Diphenylurea
Inhibitors of SARS‐CoV‐2 RdRp activity based on N,N′‐diphenylurea scaffold have been identified. Several compounds exhibit residual activity (RA) below 50% in an enzymatic assay (at 10 µM concentration) and improved aqueous solubility compared to the previously reported series of inhibitors.
Artem Chayka +11 more
wiley +1 more source
Production and crystallization of lobster muscle tropomyosin expressed in Sf9 cells
A new form of muscle tropomyosin crystal has been obtained, by employing new strategies in protein preparation and crystallization. Non‐polymerizable tropomyosin was prepared by removing 11 amino acids at the C‐terminus. The truncated tropomyosin was expressed in Sf9 insect cells by use of the baculovirus‐based expression system, to obtain highly ...
Miegel, Andrea +7 more
openaire +2 more sources
Kinetic analysis of in vitro production of wild-type Spodoptera frugiperda nucleopolyhedrovirus
In this study, the kinetic behavior of Sf9 and Sf21 cells used in the production of a baculovirus biopesticide to control the pest of corn Spodoptera frugiperda was analyzed. Kinetic variables such as maximum specific growth rate, cell productivity, mean
Andréa Farias de Almeida +3 more
doaj +1 more source
Purification and characterization of recombinant human translation initiation factor eIF3
Abstract Eukaryotic translation initiation factor 3 (eIF3) is an essential factor in protein synthesis. In mammals, it is a ~800 kDa complex composed of 12–13 subunits. Biochemical and mechanistic studies of the function of eIF3 and its individual subunits in translation require purified eIF3.
Irene Diaz‐Lopez +4 more
wiley +1 more source
Drosophila tan Encodes a Novel Hydrolase Required in Pigmentation and Vision [PDF]
Many proteins are used repeatedly in development, but usually the function of the protein is similar in the different contexts. Here we report that the classical Drosophila melanogaster locus tan encodes a novel enzyme required for two very different ...
Bernhard T Hovemann +10 more
core +4 more sources

