Results 111 to 120 of about 10,688,777 (228)

Activity‐restoring mutations in the histamine H3 receptor increase constitutive activity and reduce structural stability

Protein Science, Volume 35, Issue 1, January 2026.
Abstract The histamine H3 receptor (H3R) is a class A G protein‐coupled receptor (GPCR) that regulates neurotransmitter release in the central nervous system. Although structures of H3R in both inactive and active states have been elucidated, the functional roles of specific residues remain unclear.
Ami Nakajima, Hiroto Kaneko, Kosuke Oyama, Misumi Kuchiji, Ayane Itakura, Chiaki Arai, Mitsunori Shiroishi   +6 more
wiley   +1 more source

Cloning, Expression, and Purification of the Recombinant Hemagglutinin of Human Influenza Virus H1N1 in the Eukaryotic Insect Cells Using Baculovirus Vector

مجله دانشکده پزشکی اصفهان, 2020
Background: The H1N1 influenza virus is a highly pathogenic virus that threatens human life. Vaccination is an effective way of preventing and controlling influenza.
Niloufar Rashedi, Morteza Taghizadeh, Parisa Mohamadynejad, Mehdi Mahdavi, Reza Jalalirad   +4 more
doaj   +1 more source

Therapy of cancer by insect cells containing recombinant baculovirus encoding genes [PDF]

, 2002
Provided are compositions and methods of use for insect cells comprising baculovirus encoding non-surface expressed proteins and peptides. The claimed invention particularly relates to compositions comprising insect cells containing baculovirus that ...
Dhong, Zhongyun, Fidler, Isaiah J., Lu, Weixin   +2 more
core   +1 more source

Defining substrate specificities of human RNA capping methyltransferases through quantitative assessment of independent yet cooperative activities

Protein Science, Volume 35, Issue 1, January 2026.
Abstract Human RNA capping is critical for mRNA splicing, protection of RNA from 5′ exonucleases in the cytoplasm, and targeting to the ribosome. Human RNMT, CMTR1, and CMTR2 are RNA methyltransferases involved in the RNA capping process. They play a significant role in the proliferation and differentiation of embryonic stem cells and have been ...
Fatemeh Taherian, Mark F. Mabanglo, Taraneh Hajian, Sarah Tucker, Emilija Kalinic, Sumera Perveen, Ahmed Aman, Masoud Vedadi   +7 more
wiley   +1 more source

Chemically Defined, High-Density Insect Cell-Based Expression System for Scalable AAV Vector Production

Molecular Therapy: Methods & Clinical Development, 2020
The recombinant adeno-associated virus (AAV) vector is one of the most utilized viral vectors in gene therapy due to its robust, long-term in vivo transgene expression and low toxicity.
James H. Kurasawa, Andrew Park, Carrie R. Sowers, Rebecca A. Halpin, Andrey Tovchigrechko, Claire L. Dobson, Albert E. Schmelzer, Changshou Gao, Susan D. Wilson, Yasuhiro Ikeda   +9 more
doaj   +1 more source

Modules in the photoreceptor RGS9-1•Gβ5L GTPase-accelerating protein complex control effector coupling, GTPase acceleration, protein folding, and stability [PDF]

, 2000
RGS (regulators of G protein signaling proteins regulate G protein signaling by accelerating GTP hydrolysis, but little is known about regulation of GTPase-accelerating protein (GAP) activities or roles of domains and subunits outside the catalytic cores.
Chen, Ching-Kang, El-Hodiri, Heithem M., He, Wei, Jamrich, Milan, Lu, Lisha, Simon, Melvin I., Slep, Kevin C., Wensel, Theodore G., Zhang, Xue   +8 more
core   +1 more source

Preparation of ATP13A2 microsomes from Sf9 cells v1

, 2022
Isolate microsomes from Sf9 cells expressing ATP13A2
openaire   +1 more source

Purification of Recombinant G Proteins from Sf9 Cells by Hexahistidine Tagging of Associated Subunits

Journal of Biological Chemistry, 1995
A method is described for purification of G protein α and β subunits from Sf9 cells infected with recombinant baculoviruses. The subunit to be purified is coexpressed with an associated subunit bearing a hexahistidine tag.
T. Kozasa, A. Gilman
semanticscholar   +1 more source

Expression of active human sialyltransferase ST6GalNAcI in Escherichia coli [PDF]

, 2009
Georgios Skretas, Sean Carroll, and George Georgiou are with the Department of Chemical Engineering, University of Texas at Austin, Austin, TX 78712, USA -- George Georgiou is with the Department of Biomedical Engineering, University of Texas at Austin ...
Carroll, Sean, DeFrees, Shawn, Georgiou, George, Johnson, Karl F., Schwartz, Marc F., Skretas, Georgios   +5 more
core   +3 more sources

Evaluation of the Pichia pastoris expression system for the production of GPCRs for structural analysis

Microbial Cell Factories, 2011
Background Various protein expression systems, such as Escherichia coli (E. coli), Saccharomyces cerevisiae (S. cerevisiae), Pichia pastoris (P. pastoris), insect cells and mammalian cell lines, have been developed for the synthesis of G protein-coupled ...
Sugawara Taishi, Ito Keisuke, Tsujimoto Hirokazu, Shimamura Tatsuro, Shiroishi Mitsunori, Yurugi-Kobayashi Takami, Uemura Tomoko, Asada Hidetsugu, Nakane Takanori, Nomura Norimichi, Murata Takeshi, Haga Tatsuya, Iwata So, Kobayashi Takuya   +13 more
doaj   +1 more source