G protein beta gamma subunits synthesized in Sf9 cells. Functional characterization and the significance of prenylation of gamma [PDF]
, 1992 Heterotrimeric guanine nucleotide-binding regulatory proteins (G proteins) consist of a nucleotide-binding alpha subunit and a high- affinity complex of beta and gamma subunits.
Gilman, Alfred G. +3 more
core
High-levelexpression of functional recombinant human coagulation factor VII in insect cells [PDF]
: Recombinant coagulation factor VII (FVII) is used as a potential therapeutic intervention in hemophilia patients who produce antibodies against the coagulation factors.
AD Shapiro +30 more
core +1 more source
Altered chemomechanical coupling causes impaired motility of the kinesin-4 motors KIF27 and KIF7. [PDF]
, 2018 Kinesin-4 motors play important roles in cell division, microtubule organization, and signaling. Understanding how motors perform their functions requires an understanding of their mechanochemical and motility properties.
Blasius, T Lynne +7 more
core +2 more sources
Design, Synthesis and Bioactivity Evaluation of Novel β-carboline 1,3,4-oxadiazole Derivatives
Molecules, 2017 A series of novel β-carboline 1,3,4-oxadiazole derivatives were designed and synthesized, and the in vitro cytotoxic activity against Sf9 cells and growth inhibitory activity against Spodoptera litura were evaluated.
Zhi-Jun Zhang +3 more
doaj +1 more source
Journal of Biological Chemistry, 1997 Eukaryotic initiation factor-2B (eIF-2B) is a guanine nucleotide exchange factor (GEF) that plays a key role in the regulation of protein synthesis. In this study, we have used the baculovirus-infected Sf9 insect cell system to express and characterize ...
J. Fabian +3 more
semanticscholar +1 more source
Hsc70-induced changes in clathrin-auxilin cage structure suggest a role for clathrin light chains in cage disassembly [PDF]
, 2013 The molecular chaperone, Hsc70, together with its co-factor, auxilin, facilitates the ATP-dependent removal of clathrin during clathrin-mediated endocytosis in cells.
Brodsky, Frances M. +10 more
core +2 more sources
Reconstitution of Receptors and GTP-binding Regulatory Proteins (G Proteins) in Sf9 Cells
Journal of Biological Chemistry, 1997 The selectivity in coupling of various receptors to GTP-binding regulatory proteins (G proteins) was examined directly by a novel assay entailing the use of proteins overexpressed in Spodoptera frugiperda (Sf9) cells.
A. Barr, L. Brass, D. Manning
semanticscholar +1 more source
Expression of the Na/Pi-cotransporter Type IIb in Sf9 Cells: Functional Characterization and Purification [PDF]
, 2018 In mammals the type IIb Na/Pi-cotransporter is expressed in various tissues such as intestine, brain, lung and testis. The type IIb cotransporter shows 51% homology with the renal type IIa Na/Pi-cotransporter, for which a detailed model of the secondary ...
Biber, J., Murer, H., Radanovic, T.
core
Fibroblast Growth Factor Receptor, a Novel Receptor for Vegetative Insecticidal Protein Vip3Aa
Toxins, 2018 Vegetative insecticidal proteins (Vips), which are secreted by some Bacillus thuringiensis strains during vegetative growth, exhibit high virulence to many pests.
Kun Jiang +5 more
doaj +1 more source
Sf9 Cells Metabolism and Viability When Coinfected with Two Monocistronic Baculoviruses to Produce Rabies Virus-like Particles. [PDF]
Mol Biotechnol, 2023 Leme J +6 more
europepmc +1 more source