Lysis of mammalian and Sf9 cells. [PDF]
Methods Enzymol, 2014 Use a French press to disrupt mammalian or Sf9 cells and generate a clarified lysate for subsequent use in protein purification.
Kavran JM, Leahy DJ.
europepmc +5 more sources
Oxidative stress and DNA damage induced by spinosad exposure in Spodoptera frugiperda Sf9 cells
Food and Agricultural Immunology, 2018 Spinosad, a neurotoxic insecticide, is widely used for crop protection. In order to elucidate the effects of spinosad on oxidative stress and genotoxicity in Sf9 cells, the levels of lipid peroxidation, the activity of antioxidative enzymes, and DNA ...
Wenping Xu +4 more
doaj +2 more sources
Spodoptera frugiperda Sf9 cells as a model system to investigate the role of detoxification gene expression in response to xenobiotics [PDF]
Current Research in Insect Science, 2022 Spodoptera frugiperda (fall armyworm) is a highly destructive invasive pest that feeds on numerous crops including maize and rice. It has developed sophisticated mechanisms to detoxify xenobiotics such as secondary plant metabolites as well as manmade ...
Dries Amezian +4 more
doaj +2 more sources
Expression, purification and structural analysis of functional GABA transporter 1 using the baculovirus expression system [PDF]
Beilstein Journal of Organic Chemistry, 2017 The γ-aminobutyric acid (GABA) transporter 1 (GAT1) belongs to a family of Na+ and Cl−-coupled transport proteins and possesses 12 putative transmembrane domains. To perform structural analyses of the GAT1 protein, the GAT1/green fluorescent protein (GFP)
Jing Hu +4 more
doaj +4 more sources
Transcriptomic analysis of Spodoptera frugiperda Sf9 cells resistant to Bacillus thuringiensis Cry1Ca toxin reveals that extracellular Ca2+, Mg2+ and production of cAMP are involved in toxicity [PDF]
Biology Open, 2019 Bacillus thuringiensis (Bt) produces pore forming toxins that have been used for pest control in agriculture for many years. However, their molecular and cellular mode of action is still unclear.
Claude Castella +6 more
doaj +2 more sources
Chimeric virus-like particles carrying the CLEC17A carbohydrate-recognition domain significantly reduce Macrobrachium rosenbergii nodavirus infection in Sf9 cells [PDF]
Scientific ReportsOur previous studies demonstrated that Macrobrachium rosenbergii nodavirus (MrNV) infects and replicates in Sf9 insect cells, where fucosylated N- and O-glycans (HexNAc (Fuc)HexNAc-R or Fuc-LacdiNAc) serve as essential binding molecules for viral ...
Rueangtip Chantunmapitak +7 more
doaj +2 more sources
Genetics instability of wtAAV2 genome and AAV promoter activities in the Baculovirus/Sf9 cells system. [PDF]
PLoS ONE, 2018 The human Adeno-Associated Virus serotype 2 (wtAAV2) is a common non-pathological virus and its recombinant form (rAAV) is widely used as gene therapy vector. Although rAAVs are routinely produced in the Baculovirus/Sf9 cell system, wtAAV2 has never been
Adrien Savy +5 more
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Effects of Hormone Agonists on Sf9 Cells, Proliferation and Cell Cycle Arrest
PLoS ONE, 2011 Methoxyfenozide and methoprene are two insecticides that mimic the action of the main hormones involved in the control of insect growth and development, 20-hydroxyecdysone and juvenile hormone.
M. Giraudo +6 more
semanticscholar +8 more sources
An improved CRISPR-Cas9 protein-based method for knocking out insect Sf9 cell genes [PDF]
Applied Microbiology and BiotechnologyInsect cells are one of the uprising expression systems in the biopharmaceutical industry to produce vaccines and gene therapy vectors, but cell line development has been limited by the lack of established genetic engineering tools and genomic ...
Miguel Graça +4 more
doaj +2 more sources
Baculovirus superinfection: a probable restriction factor on the surface display of proteins for library screening. [PDF]
PLoS ONE, 2013 In addition to the expression of recombinant proteins, baculoviruses have been developed as a platform for the display of complex eukaryotic proteins on the surface of virus particles or infected insect cells.
Xiaodong Xu +6 more
doaj +3 more sources