Results 191 to 200 of about 10,688,777 (228)

The Wilson's disease protein expressed in Sf9 cells is phosphorylated

Biochemical Society Transactions, 2002
The Wilson's disease protein (WNDP), a copper transporter, is a crucial mediator of copper homoeostasis in mammalian cells. We recently found that changes in copper concentration regulate the phosphorylation level of WNDP. WNDP phosphorylation was observed in several mammalian cell lines, suggesting that a common phosphorylation pathway exists in these
S M, Vanderwerf, S, Lutsenko
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Functional Expression of His-Tagged Rhodopsin in Sf9 Insect Cells

2003
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Bosman, G.J.C.G.M., Oostrum, J. van, Breikers, G., Bovee-Geurts, P.H.M., Klaassen, C.H.W., Grip, W.J. de   +5 more
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Purification of Functional Recombinant YY1 Expressed in Sf9 Insect Cells

Protein Expression and Purification, 1995
We expressed a gene for YY1, the multifunctional mammalian transcription factor, with a recombinant baculovirus in insect cells and obtained high levels of protein in cell lysates. A simple and efficient purification method was developed. In the final step, we used DNA affinity chromatography with concatermerized sequence derived from the upstream ...
M, Momoeda, M, Kawase, N S, Young, S, Kajigaya   +3 more
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φC31 ‐Mediated cassette exchange in Sf9 insect cells for stable expression

Biotechnology Journal, 2023
AbstractInsect cells, especially Sf9 cells, are commonly used in biomanufacturing due to their advantages in high expression levels and post‐translational modification. However, the development of stable expression cell lines via random integration tended to be unstable. Site‐specific integration (SSI) is an alternative strategy. In this study, a φC31 ‐
Die Hu, Jingwen Qian, Tong Zhang, Yue Yu, Zhenhe Xu, Yuanxing Zhang, Qin Liu   +6 more
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Activation of recombinant trp by thapsigargin in Sf9 insect cells

American Journal of Physiology-Cell Physiology, 1994
The mammalian protein responsible for Ca2+ release-activated current (Icrac) may be homologous to the Drosophila protein designated trp. Thus the activity of trp, and another Drosophila protein designated trp-like or trpl, may be linked to depletion of the internal Ca2+ store via the so-called capacitative Ca2+ entry mechanism. To test this hypothesis,
L, Vaca, W G, Sinkins, Y, Hu, D L, Kunze, W P, Schilling   +4 more
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Gbetagamma-copurified lipid kinase impurity from Sf9 cells.

Protein and peptide letters, 2009
G-protein betagamma dimers are prime regulators transmitting extracellular signals to wide-ranging cellular effectors including phosphoinositide 3-kinase (PI3K) isoforms beta and gamma. Recombinant Gbetagamma purified from Sf9 cells via metal-affinity and anion exchange chromatography exhibited a wortmannin-insensitive phospholipid kinase activity that
Aliaksei, Shymanets, Mohammad Reza, Ahmadian, Bernd, Nürnberg   +2 more
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Comparative characterization of cell death between Sf9 insect cells and hybridoma cultures

Biotechnology and Bioengineering, 2001
Physiological cell death (PCD) in Sf9 insect cell batch cultures was comprehensively characterized using simultaneous determinations of qualitative and quantitative assays, including agarose gel electrophoresis, confocal, epifluorescence, and transmission electron microscopy, and DNA content by flow cytometry.
A, Meneses-Acosta, R, Mendonça, H, Merchant, L, Covarrubias, O, Ramírez   +4 more
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Endogenous K+/H+ Exchange Activity in the Sf9 Insect Cell Line

Biochemistry, 1995
The fluorescent pH indicator 2',7'-bis(carboxyethyl)-5,6-carboxyfluorescein (BCECF) was used to investigate changes in the intracellular pH (pHi) of individual Sf9 cells in response to changes in the composition of the external medium. Under standard conditions, the resting pHi was 0.2-0.3 unit lower than the extracellular pH (6.5).
Vachon, Vincent, Paradis, Marie-Josee, Marsolais, Mireille, Schwartz, Jean-Louis, Laprade, Raynald   +4 more
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Functional expression of solubleicam-1 by baculovirus-infected Sf9 cells

Biochemical and Biophysical Research Communications, 1992
Inflammation, metastasis and ischemia are processes that require lymphocyte or leukocyte cell recognition and adherence to endothelial counter receptors such as ICAM-1. Mapping the sites of interaction of ICAM-1 with LFA-1, the receptor for ICAM-1 on lymphocytes, may lead to the design of novel inhibitors of inflammation or metastasis.
R R, Cobb, J S, Dubins, J, Warner, L, Molony   +3 more
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Assays of Recombinant Adenylyl Cyclases Expressed in Sf9 Cells

2003
This chapter outlines procedures for the expression of mammalian membrane-bound adenylyl cyclases (AC) in Sf9 cells and subsequent in vitro methods for assessing the activity of these cyclases. Membrane preparations derived from this overexpression system provide homogeneous sources of mammalian AC because AC that are endogenously expressed in Sf9 ...
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