Results 201 to 210 of about 10,688,777 (228)

Eextracellular Histone H4 from SF9 Cells Is Antimicrobial

2005
Spodoptera frugiperda Sf9 cells produces an extracellular, truncated form of histone H4 (H4-N12). H4-N12 was suspected to have antimicrobial effects. To investigate this, conditioned medium from Sf9 cell cultures was concentrated and fractioned on a gel filtration column. The fractions containing H4-N12, were collected, pooled and the buffer exchanged (
K. Calles, E. Lindskog, Å. Nossed, I. Svensson, L. Häggström   +4 more
openaire   +1 more source

Radiation responses of Sf9, a highly radioresistant lepidopteran insect cell line

International Journal of Radiation Biology, 2004
Lepidopteran insect cells are known to exhibit very high radioresistance. Although very effective DNA excision-repair has been proposed as a contributing factor, a detailed understanding of insect cell radiation responses has not yet been obtained. Therefore, the study was carried out to understand the in vitro radiation responses of Sf9 lepidopteran ...
S, Chandna, B S, Dwarakanath, R K, Seth, D, Khaitan, J S, Adhikari, V, Jain   +5 more
openaire   +2 more sources

Characterization of human 5-HT1 receptors expressed in Sf9 insect cells

European Journal of Pharmacology: Molecular Pharmacology, 1994
Four human 5-HT receptor subtypes (5-HT1A, 5-HT1D alpha, 5-HT1D beta and 5-HT1E) have been expressed in Sf9 insect cells. All four human 5-hydroxytryptamine receptors produced by Sf9 cells had the expected pharmacological properties. Surprisingly, levels of expression of these receptors were relatively low (1-5 pmol/mg protein).
E M, Parker, D A, Grisel, L G, Iben, H P, Nowak, C D, Mahle, F D, Yocca, G T, Gaughan   +6 more
openaire   +2 more sources

Silkworm hemolymph as a potent inhibitor of apoptosis in Sf9 cells

Biochemical and Biophysical Research Communications, 2002
We have previously shown that silkworm hemolymph exhibits anti-apoptotic activity against baculovirus-induced Sf9 cell apoptosis. In this study, using various chemical inducers, such as actinomycin D, camptothecin, and staurosporine, we found that silkworm hemolymph inhibits insect cell apoptosis induced not only by baculovirus but also by chemical ...
Won Jong, Rhee, Eun Jeong, Kim, Tai Hyun, Park   +2 more
openaire   +2 more sources

Homogeneous Purification of Human Recombinant GST–Akt/PKB from Sf9 Cells

Protein Expression and Purification, 1999
The plethora of extracellular stimuli modulating the status of a cell results in the engagement of relatively few pathways responsible for transducing signals to the interior of the cell. One such pathway is the activation of phosphatidylinositol 3-kinase (PI-3K), which results in the generation of a membrane-restricted second messenger ...
D, Fabbro, D, Batt, P, Rose, B, Schacher, T M, Roberts, S, Ferrari   +5 more
openaire   +2 more sources

Solubilization of muscarinic receptor subtypes from baculovirus infected sf9 insect cells

Biochemical Pharmacology, 1994
Five different subtypes (human m1, m2, m5 and rat m3, m4) of muscarinic acetylcholine receptors (mAChR) were produced in insect Sf9 cells by infection with recombinant baculoviruses. N-[3H]methylscopolamine ([3H]NMS) has a similar affinity to each of these mAChR subtypes in cell membranes, while pirenzepine, 11-((2-[(diethylamino)methyl]-1-piperidinyl ...
A, Rinken, K, Kameyama, T, Haga, L, Engström   +3 more
openaire   +2 more sources

[Localization of AcMNPV NLA genes in Sf9 cells].

Bing du xue bao = Chinese journal of virology, 2012
Nuclear actin which plays a key role in many nucleic processes has become a research hotspot. Baculovirus is the only reported pathogen using nuclear actin to replicate and proliferate. However, little is known about the mechanism of monomeric G-actin accumulation within nuclei of baculovirus-infected cells. It has been reported that AcMNPV ie-1, pe38,
Qian, Wang, Ji-Zheng, Chen, Yun, Wang, Xue-Jun, Wang, Xin-Wen, Chen   +4 more
openaire   +1 more source

Purification and Characterization of Aspartic Protease Derived from Sf9 Insect Cells

Bioscience, Biotechnology, and Biochemistry, 2010
An aspartic protease that is significantly produced by baculovirus-infected Spodoptera frugiperda Sf9 insect cells was purified to homogeneity from a growth medium. To monitor aspartic protease activity, an internally quenched fluoresce (IQF) substrate specific to cathepsin D was used.
Takeshi, Gotoh, Hiroki, Ono, Ken-Ichi, Kikuchi, Satoru, Nirasawa, Saori, Takahashi   +4 more
openaire   +2 more sources

Detection of active infection of Sf9 insect cells by recombinant baculoviruses

Journal of Virological Methods, 1997
Production of different recombinant proteins in baculovirus AcMNPV (BV)-infected cells may be facilitated by the availability of immunoassays to monitor active infection of Sf9 insect cells. To this end, two hybridomas secreting mouse monoclonal antibodies (mAbs) were established to different BV-related products. The proteins recognized by mAb SM22 and
S M, Mariani, P H, Krammer
openaire   +2 more sources

Properties of single Drosophila Trpl channels expressed in Sf9 insect cells

American Journal of Physiology-Cell Physiology, 1997
The transient receptor potential (trp)-like (trpl) gene is thought to encode an ion channel important for signal transduction in Drosophila photoreceptor cells. Consistent with this hypothesis, heterologous expression of the trpl-encoded protein (Trpl) is associated with the appearance of an outwardly rectifying, nonselective cation current.
D L, Kunze, W G, Sinkins, L, Vaca, W P, Schilling   +3 more
openaire   +2 more sources