Ethanol Stimulates Expression of Functional H+,K+-ATPase in SF9 Cells [PDF]
Biochemical and Biophysical Research Communications, 1995 The baculovirus expression system is suitable for functional expression of gastric H+,K(+)-ATPase. Expression of functional H+,K(+)-ATPase in Sf9 cells is accompanied by synthesis of large amounts of non-functional subunits. When H+,K(+)-ATPase is synthesised in the presence of 150-250 mM ethanol in the culture medium, two to threefold higher levels of
Klaassen, C.H.W. +2 more
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Viruses, 2016 At present, the details of lamina alterations after baculovirus infection remain elusive. In this study, a lamin gene in the Sf9 cell line of Spodoptera frugiperda was cloned.
Wenqiang Wei +8 more
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Comparing cytotoxicity of propoxur and Nepeta crispa (Lamiales: Lamiaceae) essential oil against invertebrate (Sf9) and vertebrate (L929) cell lines [PDF]
Veterinary World, 2019 Background and Aim: Attempts to use the plant products are to be an appropriate option due to substantial concerns about human health and environmental problems of using synthetic pesticides. Therefore, the cytotoxicity of Nepeta crispa essential oil was
Amirhossein Zahirnia +4 more
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Expression of functional recombinant human tissue transglutaminase (TG2) using the bac-to-bac baculovirus expression system [PDF]
, 2016 Purpose: Tissue transglutaminase (TG2) is a unique multifunctional enzyme. The enzyme possesses enzymatic activities such as transamidation/crosslinking and non-enzymatic functions such as cell migration and signal transduction.
Azari, S., Kalhor, H.R., Yazdani, Y.
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PLoS ONE, 2016 Sf9, a cell line derived from Spodoptera frugiperda, is an ideal model organism for studying insect apoptosis. The first notable study that attempted to identify the apoptotic pathway in Sf9 was performed in 1997 and included the discovery of Sf-caspase ...
Zhouning Yang +5 more
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Mitochondria and Lysosomes Participate in Vip3Aa-Induced Spodoptera frugiperda Sf9 Cell Apoptosis
Toxins, 2020 Vip3Aa, a soluble protein produced by certain Bacillus thuringiensis strains, is capable of inducing apoptosis in Sf9 cells. However, the apoptosis mechanism triggered by Vip3Aa is unclear.
Xiaoyue Hou +8 more
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Interaction of the Xanthine Nucleotide Binding Goα Mutant with G Protein-coupled Receptors [PDF]
, 1998 We constructed a double mutant version of the α subunit of Go that was regulated by xanthine nucleotides instead of guanine nucleotides (GoαX). We investigated the interaction between GoαX and G protein-coupled receptors in vitro.
Simon, Melvin I., Yu, Bo
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Molecules, 2011 Many studies on intracellular calcium ([Ca2+]i) and intracellular pH (pHi) have been carried out due to their importance in regulation of different cellular functions. However, most of the previous studies are focused on human or mammalian cells.
Yan-Bo Zhang +4 more
doaj +1 more source
Identification and characterization of the Spodoptera Su(var) 3-9 histone H3K9 trimethyltransferase and its effect in AcMNPV infection. [PDF]
PLoS ONE, 2013 Histone H3-lysine(9) (H3K9) trimethyltransferase gene Su(var) 3-9 was cloned and identified in three Spodoptera insects, Spodopterafrugiperda (S. frugiperda), S. exigua and S. litura.
Binbin Li +3 more
doaj +1 more source
Studies on processing, particle formation, and immunogenicity of the HIV-1 gag gene product: a possible component of a HIV vaccine [PDF]
, 1992 Antigens in a particulate conformation were shown to be highly immunogenic in mammals. For this reason, the particle forming capacity of derivatives of the HIV-1 group specific core antigen p55 gag was assayed and compared dependent on various expression
Deby, G. +7 more
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