Cell-Cycle Perturbation in Sf9 Cells Infected withAutographa californicaNucleopolyhedrovirus
Virology, 1999 Flow cytometry analysis of the cell-cycle progression was performed in Sf9 cells infected with Autographa californica nucleopolyhedrovirus (AcNPV) in the cultures partially synchronized by aphidicolin exposure and deprivation. Cells infected with AcNPV during the G1 phase progressed and were arrested in the S phase in the 4 h following the infection ...
Ikeda, Motoko, Kobayashi, Michihiro
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Evidence for the existence of Dm0-like Lamin in Sf9 cells
浙江大学学报. 农业与生命科学版, 2015 The nuclear membrane of mammalian cells was composed of inner nuclear membrane, outer nuclear membrane and perinuclear space. The lamina was localized under the nucleoplasm face of inner nuclear membrane. It has been known that the lamina was distributed
Wei Wenqiang, Ji Shaoping, Zhang Yinyan
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The mitochondria-mediate apoptosis of Lepidopteran cells induced by azadirachtin. [PDF]
PLoS ONE, 2013 Mitochondria have been shown to play an important role in apoptosis using mammalian cell lines. However, this seems not to be the case in Drosophila, an insect model organism; thus more in-depth studies of insect cell apoptosis are necessary.
Jingfei Huang +3 more
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Gad65 is recognized by t-cells, but not by antibodies from nod-mice [PDF]
, 1994 Since the 64kDa-protein glutamic acid decarboxylase (GAD) is one of the major autoantigens in T-cell mediated Type 1 diabetes, its relevance as a T-cell antigen needs to be clarified. After isolation of splenic T-cells from non-obese diabetic (NOD) mice,
Alzari PM +28 more
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DSP-PP precursor protein cleavage by tolloid-related-1 protein and by bone morphogenetic protein-1.
PLoS ONE, 2012 Dentin sialoprotein (DSP) and phosphophoryn (PP), acidic proteins critical to dentin mineralization, are translated from a single transcript as a DSP-PP precursor that undergoes specific proteolytic processing to generate DSP and PP.
Helena H Ritchie +4 more
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Production and characterization of novel recombinant adeno-associated virus replicative-form genomes: a eukaryotic source of DNA for gene transfer. [PDF]
PLoS ONE, 2013 Conventional non-viral gene transfer uses bacterial plasmid DNA containing antibiotic resistance genes, cis-acting bacterial sequence elements, and prokaryotic methylation patterns that may adversely affect transgene expression and vector stability in ...
Lina Li +9 more
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Binding specificity of ostreolysin A6 towards Sf9 insect cell lipids
Biochimica et Biophysica Acta (BBA) - Biomembranes, 2020 Oyster mushrooms (Pleurotus spp.) have recently been shown to produce insecticidal bi-component protein complexes based on the aegerolysin proteins. A role for these proteins is thus indicated for defence and protection of the mushroom, and we propose their use as new environmentally friendly bioinsecticides.
Novak M. +7 more
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Inhibition of Subsets of G Protein-coupled Receptors by Empty Mutants of G Protein α Subunits in Go, G11, and G16 [PDF]
, 2000 We previously reported that the xanthine nucleotide binding Goα mutant, GoαX, inhibited the activation of Gi-coupled receptors. We constructed similar mutations in G11α and G16α and characterized their nucleotide binding and receptor interaction.
Gu, Lingjie, Simon, Melvin I., Yu, Bo
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Protease-mediated maturation of M-PMV reverse transcriptase into a functional heterodimer. [PDF]
Protein SciAbstract Reverse transcriptase (RT) of retroviruses orchestrates viral replication, yet its structural diversity remains poorly understood. Well‐studied RTs, such as those from HIV‐1, murine leukemia virus, and avian myeloblastosis virus, were characterized decades ago, but less prominent retroviruses have escaped detailed analysis.
Kapisheva M +6 more
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Both Ligand- and Cell-Specific Parameters Control Ligand Agonism in a Kinetic Model of G Protein–Coupled Receptor Signaling [PDF]
, 2007 G protein–coupled receptors (GPCRs) exist in multiple dynamic states (e.g., ligand-bound, inactive, G protein–coupled) that influence G protein activation and ultimately response generation. In quantitative models of GPCR signaling that incorporate these
Kinzer-Ursem, Tamara L. +1 more
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