Results 71 to 80 of about 10,688,777 (228)

Gut Microbe‐Driven Resistance Mechanisms in Propylea Japonica: Insights from Horizontal Gene Transfer and Oxidative Phosphorylation

Advanced Science, EarlyView.
Acinetobacter regulates dinotefuran tolerance in Propylea japonica by mediating the expression of the horizontally transferred gene PjDUF1. Abstract Insect–microbial symbiont relationships are widespread in nature and often involve lateral gene transfer.
Ningbo HuangFu, Xiangzhen Zhu, Zhijuan Tang, Li Wang, Kaixin Zhang, Dongyang Li, Jichao Ji, Jinjie Cui, Zhaojiang Guo, Junyu Luo, Xueke Gao   +10 more
wiley   +1 more source

Immunogenicity of HGV NS5 protein expressed from Sf9 insect cells

World Journal of Gastroenterology, 2001
Although reliable assays for the detection of hepatitis C virus and E virus became available, still 10%-20% hepatitis are not caused by hepatitisA-E virus[1-3]. In 1996, two research groups isolated this agent independently and almost simultaneously and named hepatitis G virus and GB virus C, respectively[4-7].
H, Ren, F L, Zhu, S Y, Zhu, Y, Song, Z T, Qi   +4 more
openaire   +2 more sources

Receptor-mediated activation of recombinant Trpl expressed in Sf9 insect cells [PDF]

Biochemical Journal, 1995
The Drosophila proteins, Trp and Trpl, are suggested to be cation channels responsible for depolarization of the receptor potential associated with stimulation of insect photoreceptor cells by light. Consistent with this hypothesis, we recently showed that recombinant Trpl forms Ca(2+)- and Ba(2+)-permeable non-selective cation channels when expressed ...
Y, Hu, W P, Schilling
openaire   +2 more sources

Identification of novel linear epitopes in P72 protein of African swine fever virus recognized by monoclonal antibodies

Frontiers in Microbiology, 2022
African swine fever (ASF) is one of the highly contagious and lethal diseases among domestic pigs and wild boars. The capsid protein P72 of African swine fever virus (ASFV) is very important for the diagnosis and vaccine development. However, the epitope
Dan Yin, Dan Yin, Dan Yin, Renhao Geng, Renhao Geng, Renhao Geng, Hongxia Shao, Hongxia Shao, Hongxia Shao, Jianqiang Ye, Jianqiang Ye, Jianqiang Ye, Kun Qian, Kun Qian, Kun Qian, Hongjun Chen, Aijian Qin, Aijian Qin, Aijian Qin   +18 more
doaj   +1 more source

Molecular bases of insect odorant receptor function: specificity and evolution

Biological Reviews, EarlyView.
ABSTRACT Insect odorant receptors (ORs) are a class of chemoreceptors that insects use to detect volatile cues in their environment. In recent years, major advances in the field of structural biology have made it possible to obtain the first structures of insect ORs.
Zibo Li, Abhinob Baruah, Sridevi Bhamidipati, Nicolas Montagné, Emmanuelle Jacquin‐Joly, Camille Meslin   +5 more
wiley   +1 more source

Purification, Fluorescent Labeling, and Detyrosination of Mammalian Cell Tubulin for Biochemical Assays

Cytoskeleton, EarlyView.
ABSTRACT Microtubules play essential roles in numerous cellular processes. All microtubules are built from the protein tubulin, yet individual microtubules can differ spatially and temporally due to their tubulin isotype composition and post‐translational modifications (PTMs).
Ezekiel C. Thomas, Yang Yue, Morgan L. Pimm, Takashi Hotta, Ryoma Ohi, Kristen J. Verhey   +5 more
wiley   +1 more source

Characterization of Endogenous Retroviral-like Particles Expressed from the Spodoptera frugiperda Sf9 Cell Line

Viruses
The Spodoptera frugiperda Sf9 insect cell line is used in the baculovirus expression vector system for the development of various viral vaccines and some gene therapy products.
Hailun Ma, Eunhae H. Bae, Pei-Ju Chin, Arifa S. Khan   +3 more
doaj   +1 more source

Ao38, a new cell line from eggs of the black witch moth, Ascalapha odorata (Lepidoptera: Noctuidae), is permissive for AcMNPV infection and produces high levels of recombinant proteins

BMC Biotechnology, 2010
Background The insect cell line is a critical component in the production of recombinant proteins in the baculovirus expression system and new cell lines hold the promise of increasing both quantity and quality of protein production. Results Seventy cell
Zhang Sheng, Hashimoto Yoshifumi, Blissard Gary W   +2 more
doaj   +1 more source

Cytoplasmic islet cell antibodies recognize distinct islet antigens in IDDM but not in stiff man syndrome [PDF]

, 1993
Cytoplasmic islet cell antibodies are well-established predictive markers of IDDM. Although target molecules of ICA have been suggested to be gangliosides, human monoclonal ICA of the immunoglobulin G class (MICA 1-6) produced from a patient with newly ...
Meinck, Hans-Michael, Northemann, Wolfgang, Richter, Wiltrud, Scherbaum, Werner A., Seißler, Jochen, Wolfahrt, S.   +5 more
core   +1 more source

Protocol for Reconstituting Adaptor‐Mediated Activation of Full‐Length Kinesin‐1

Cytoskeleton, EarlyView.
ABSTRACT Kinesin‐1 is a member of the kinesin superfamily that plays an essential role in intracellular cargo transport. In the absence of cargo, Kinesin‐1 exhibits low motor activity due to autoinhibition. Multiple studies have demonstrated that adaptor proteins, which link cargos to Kinesin‐1, can activate Kinesin‐1 by releasing the autoinhibition ...
Haruka Masumoto, Kyoko Chiba
wiley   +1 more source