Results 81 to 90 of about 10,688,777 (228)
Baculovirus infection induces disruption of the nuclear lamina
Scientific Reports, 2017 Baculovirus nucleocapsids egress from the nucleus primarily via budding at the nuclear membrane. The nuclear lamina underlying the nuclear membrane represents a substantial barrier to nuclear egress. Whether the nuclear lamina undergoes disruption during
Xiaomei Zhang +5 more
doaj +1 more source
Exploring the Comprehensive Kozak Sequence Landscape for AAV Production in Sf9 System
Viruses, 2023 The widespread successful use of recombinant Adeno-associated virus (rAAV) in gene therapy has driven the demand for scale-up manufacturing methods of vectors with optimized yield and transduction efficiency.
Oleksandr Kondratov, Sergei Zolotukhin
doaj +1 more source
Interaction between functional domains of Bacillus thuringiensis insecticidal crystal proteins [PDF]
, 1999 Interactions among the three structural domains of Bacillus 1huringiensis Cn.l toxins %~ere investigated by functional analysis of chinieric proteins.
Bosch, D. +6 more
core
PLoS ONE, 2018 Nosema bombycis is a destructive, obligate intracellular parasite of the Bombyx mori. In this study, a single-chain variable fragment (scFv) dependent technology is developed for the purpose of inhibiting parasite proliferation in insect cells.
Yukang Huang +10 more
semanticscholar +1 more source
Cytoskeleton, EarlyView.ABSTRACT Cooperativity between cytoskeletal proteins is crucial for spatiotemporal coordination in biological processes, like oogenesis. In mammalian and Drosophila oogenesis, proper assembly and function of actin networks require coordination between actin assembly factors Spire and formins, as well as actin‐associated proteins like myosins and Rab ...
Joseph Y. Ong +7 more
wiley +1 more source
Cytoskeleton, EarlyView.ABSTRACT Microtubules are noncovalent polymers assembled from α/β tubulin dimers. Their structure, dynamics and interaction with effectors are regulated through the expression of diverse tubulin isotypes and chemically diverse posttranslational modifications, also known as the “tubulin code.” Understanding the biophysical correlates between tubulin ...
Jiayi Chen +2 more
wiley +1 more source
Expression and characterization of two STAT isoforms from Sf9 cells
Developmental & Comparative Immunology, 2008 In invertebrates, the JAK-STAT signaling pathway is involved in the anti-bacterial response and is part of an anti-viral response in Drosophila. In this study, we show that two STAT transcripts are generated by alternative splicing and encode two isoforms of Sf-STAT with different C-terminal ends. These two isoforms were produced and purified using the
Maw-Sheng, Yeh +8 more
openaire +2 more sources
Expression and purification of human perforin by sf9 insect cells [PDF]
Jichu yixue yu linchuang, 2020 Objective To optimize the purification method of human perforin. Methods Perforin with His tag was expressed by sf9 insect cells, and then protein affinity chromatography was performed by Ni column device.
CHEN Feng-ye, LIU Meng-yu, ZHOU Ya-bo, LIANG Xiao-yu, HUANG Bo, XIE Jing
doaj
ADENYLYL CYCLASE TYPE 9: REGULATION AND CARDIAC FUNCTION [PDF]
, 2018 Abnormalities in cardiac stress signaling underlie a number of cardiovascular diseases (e.g. arrhythmias and heart failure). Cardiac stress signaling pathways normally integrate signals from the sympathetic nervous system to promote efficient contraction
Baldwin, Tanya A., Baldwin, Tanya A.
core +1 more source
Integrative Zoology, EarlyView.This work focused on arowana fishes (Teleostei, Osteoglossiformes) as a model; their widespread intercontinental distribution and basal phylogenetic position within Teleostei make them a compelling model for evolutionary research, especially in the realm of satDNA molecular evolution. Through the integration of genomic and chromosomal data, we analyzed
Fernando Henrique Santos de Souza +9 more
wiley +1 more source