Results 11 to 20 of about 5,672,019 (265)

Establishment of Babesia bovis In Vitro Culture Using Medium Free of Animal Products

Pathogens, 2021
Babesia bovis, an etiological agent of bovine babesiosis, causes a significant burden to the cattle industry worldwide. The most efficient method to mitigate bovine babesiosis is a live vaccine produced by serial passage in splenectomized cattle. However,
Jesús A. Álvarez Martínez, Julio V. Figueroa Millán, Massaro W. Ueti, Carmen Rojas-Martínez   +3 more
doaj   +2 more sources

Innovative Alternatives for Continuous In Vitro Culture of Babesia bigemina in Medium Free of Components of Animal Origin

Pathogens, 2020
In this study, we report Babesia bigemina proliferation in culture medium free of components of animal origin supplemented with a lipid mixture. Babesia bigemina continuously proliferated in VP-SFM with a higher percent parasitized erythrocyte as compare
Jesús A. Álvarez Martínez, Julio V. Figueroa Millán, Massaro W. Ueti, Carmen Rojas-Martínez   +3 more
doaj   +2 more sources

A Method to Generate and Rescue Recombinant Adenovirus Devoid of Replication-Competent Particles in Animal-Origin-Free Culture Medium

Viruses, 2023
Adenoviruses are promising vectors for vaccine production and gene therapy. Despite all the efforts in removing animal-derived components such as fetal bovine serum (FBS) during the production of adenovirus vector (AdV), FBS is still frequently employed ...
Seyyed Mehdy Elahi, Jennifer Jiang, Nazila Nazemi-Moghaddam, Rénald Gilbert   +3 more
doaj   +2 more sources

Development of the Animal Empathy Scale Short Form

Psikiyatride Güncel Yaklaşımlar, 2023
This study aimed to develop the short form of the Animal Empathy Scale (AES-SF). To this end, the study first conducted the Turkish validity and reliability analyses of the long scale and the selection of the items for the short form.
Nur Okutan
doaj   +2 more sources

Identification and Characterization of Planktonic Biofilm-Like Aggregates in Infected Synovial Fluids From Joint Infections

Frontiers in Microbiology, 2020
Recent in vitro studies reported the exceptional ability of some bacterial species to form biofilm-like aggregates in human and animal synovial fluids (SF), but evidences from infected clinical samples are still lacking.
Alessandro Bidossi, Marta Bottagisio, Paolo Savadori, Elena De Vecchi   +3 more
doaj   +2 more sources

Replacing highly degradable starch with soluble fiber reduces the accumulation of trans-10 C18:1 in continuous culture fermenters with a basal level of soybean oil

Frontiers in Animal Science
Evaluation of the starch degradability (ShD) and the starch level in a ration is necessary for determining how a ration will affect performance in lactating dairy cows. Ingredients high in starch with high rates of degradability can lead to a decrease in
Louisa Koch, Brandon Koch, Thomas C. Jenkins, Wilmer Cuervo, Gustavo J. Lascano   +4 more
doaj   +2 more sources

Cryopreservation of jaguar (<i>Panthera onca</i>) fibroblast-like cells in straws and cryovials: a comparison of rapid and slow-freezing protocols. [PDF]

Anim Reprod
Somatic cells serve as essential genetic resources for biobanking. Traditionally, jaguar fibroblast-like cells are frozen in cryovials using slow-freezing protocols.
Polizelle SR, Navarezi IF, da Silva MCC, Vieira GC, Gaeth LM, Brandão-Souza LS, Acacio BR, Jorge-Neto PN, Silva EVDCE, de Deco-Souza T, Pereira AF, de Araujo GR.   +11 more
europepmc   +2 more sources

Meiotic and developmental competence of growing pig oocytes derived from small antral follicles is enhanced in culture medium containing FGF2, LIF, and IGF1 (FLI medium)

Journal of Ovarian Research
Background Oocytes of large animal species isolated from small ovarian follicles (
Alexandra Rosenbaum Bartková, Lucie Němcová, Veronika Kinterová, Dominika Radová, František Strejček, Tereza Toralová, Jozef Laurinčík, Radek Procházka   +7 more
doaj   +2 more sources

Optimising and adapting perfusion feeds in serum-free medium to intensify CAR-T cell expansion in stirred-tank bioreactors

Frontiers in Bioengineering and Biotechnology
The ex vivo expansion of autologous chimeric antigen receptor (CAR) T cells to reach a therapeutic dose significantly prolongs manufacturing time and increases overall costs.
Pierre Springuel, Tiffany Hood, Fern Slingsby, Timo Schmidberger, Nicola Bevan, Noushin Dianat, Julia Hengst, Qasim A. Rafiq   +7 more
doaj   +2 more sources

Response of Dermal Fibroblasts to Biochemical and Physical Cues in Aligned Polycaprolactone/Silk Fibroin Nanofiber Scaffolds for Application in Tendon Tissue Engineering

Nanomaterials, 2017
Silk fibroin (SF) and fiber alignment were introduced into polycaprolactone (PCL)-based electrospun nanofibers as chemical and physical cues for tendon tissue engineering applications.
Chih-Hao Chen, Shih-Hsien Chen, Chang-Yi Kuo, Meng-Lun Li, Jyh-Ping Chen   +4 more
doaj   +2 more sources